Cargando…

The octamer is the major form of CENP-A nucleosomes at human centromeres

The centromere is the chromosomal locus that ensures fidelity in genome transmission at cell division. Centromere protein A (CENP-A) is a histone H3 variant that specifies centromere location independently of DNA sequence. Conflicting evidence has emerged regarding the histone composition and stoich...

Descripción completa

Detalles Bibliográficos
Autores principales: Hasson, Dan, Panchenko, Tanya, Salimian, Kevan J., Salman, Mishah U., Sekulic, Nikolina, Alonso, Alicia, Warburton, Peter E., Black, Ben E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760417/
https://www.ncbi.nlm.nih.gov/pubmed/23644596
http://dx.doi.org/10.1038/nsmb.2562
_version_ 1782282766773125120
author Hasson, Dan
Panchenko, Tanya
Salimian, Kevan J.
Salman, Mishah U.
Sekulic, Nikolina
Alonso, Alicia
Warburton, Peter E.
Black, Ben E.
author_facet Hasson, Dan
Panchenko, Tanya
Salimian, Kevan J.
Salman, Mishah U.
Sekulic, Nikolina
Alonso, Alicia
Warburton, Peter E.
Black, Ben E.
author_sort Hasson, Dan
collection PubMed
description The centromere is the chromosomal locus that ensures fidelity in genome transmission at cell division. Centromere protein A (CENP-A) is a histone H3 variant that specifies centromere location independently of DNA sequence. Conflicting evidence has emerged regarding the histone composition and stoichiometry of CENP-A nucleosomes. Here we show that the predominant form of the CENP-A particle at human centromeres is an octameric nucleosome. CENP-A nucleosomes are very highly phased on α-satellite 171 bp monomers at normal centromeres, and also display strong positioning at neocentromeres. At either type of functional centromere, CENP-A nucleosomes exhibit similar DNA wrapping behavior as octameric CENP-A nucleosomes reconstituted with recombinant components, having looser DNA termini than those on their conventional counterparts containing canonical H3. Thus, the fundamental unit of the chromatin that epigenetically specifies centromere location in mammals is an octameric nucleosome with loose termini.
format Online
Article
Text
id pubmed-3760417
institution National Center for Biotechnology Information
language English
publishDate 2013
record_format MEDLINE/PubMed
spelling pubmed-37604172013-12-01 The octamer is the major form of CENP-A nucleosomes at human centromeres Hasson, Dan Panchenko, Tanya Salimian, Kevan J. Salman, Mishah U. Sekulic, Nikolina Alonso, Alicia Warburton, Peter E. Black, Ben E. Nat Struct Mol Biol Article The centromere is the chromosomal locus that ensures fidelity in genome transmission at cell division. Centromere protein A (CENP-A) is a histone H3 variant that specifies centromere location independently of DNA sequence. Conflicting evidence has emerged regarding the histone composition and stoichiometry of CENP-A nucleosomes. Here we show that the predominant form of the CENP-A particle at human centromeres is an octameric nucleosome. CENP-A nucleosomes are very highly phased on α-satellite 171 bp monomers at normal centromeres, and also display strong positioning at neocentromeres. At either type of functional centromere, CENP-A nucleosomes exhibit similar DNA wrapping behavior as octameric CENP-A nucleosomes reconstituted with recombinant components, having looser DNA termini than those on their conventional counterparts containing canonical H3. Thus, the fundamental unit of the chromatin that epigenetically specifies centromere location in mammals is an octameric nucleosome with loose termini. 2013-05-05 2013-06 /pmc/articles/PMC3760417/ /pubmed/23644596 http://dx.doi.org/10.1038/nsmb.2562 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hasson, Dan
Panchenko, Tanya
Salimian, Kevan J.
Salman, Mishah U.
Sekulic, Nikolina
Alonso, Alicia
Warburton, Peter E.
Black, Ben E.
The octamer is the major form of CENP-A nucleosomes at human centromeres
title The octamer is the major form of CENP-A nucleosomes at human centromeres
title_full The octamer is the major form of CENP-A nucleosomes at human centromeres
title_fullStr The octamer is the major form of CENP-A nucleosomes at human centromeres
title_full_unstemmed The octamer is the major form of CENP-A nucleosomes at human centromeres
title_short The octamer is the major form of CENP-A nucleosomes at human centromeres
title_sort octamer is the major form of cenp-a nucleosomes at human centromeres
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760417/
https://www.ncbi.nlm.nih.gov/pubmed/23644596
http://dx.doi.org/10.1038/nsmb.2562
work_keys_str_mv AT hassondan theoctameristhemajorformofcenpanucleosomesathumancentromeres
AT panchenkotanya theoctameristhemajorformofcenpanucleosomesathumancentromeres
AT salimiankevanj theoctameristhemajorformofcenpanucleosomesathumancentromeres
AT salmanmishahu theoctameristhemajorformofcenpanucleosomesathumancentromeres
AT sekulicnikolina theoctameristhemajorformofcenpanucleosomesathumancentromeres
AT alonsoalicia theoctameristhemajorformofcenpanucleosomesathumancentromeres
AT warburtonpetere theoctameristhemajorformofcenpanucleosomesathumancentromeres
AT blackbene theoctameristhemajorformofcenpanucleosomesathumancentromeres
AT hassondan octameristhemajorformofcenpanucleosomesathumancentromeres
AT panchenkotanya octameristhemajorformofcenpanucleosomesathumancentromeres
AT salimiankevanj octameristhemajorformofcenpanucleosomesathumancentromeres
AT salmanmishahu octameristhemajorformofcenpanucleosomesathumancentromeres
AT sekulicnikolina octameristhemajorformofcenpanucleosomesathumancentromeres
AT alonsoalicia octameristhemajorformofcenpanucleosomesathumancentromeres
AT warburtonpetere octameristhemajorformofcenpanucleosomesathumancentromeres
AT blackbene octameristhemajorformofcenpanucleosomesathumancentromeres