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The octamer is the major form of CENP-A nucleosomes at human centromeres
The centromere is the chromosomal locus that ensures fidelity in genome transmission at cell division. Centromere protein A (CENP-A) is a histone H3 variant that specifies centromere location independently of DNA sequence. Conflicting evidence has emerged regarding the histone composition and stoich...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760417/ https://www.ncbi.nlm.nih.gov/pubmed/23644596 http://dx.doi.org/10.1038/nsmb.2562 |
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author | Hasson, Dan Panchenko, Tanya Salimian, Kevan J. Salman, Mishah U. Sekulic, Nikolina Alonso, Alicia Warburton, Peter E. Black, Ben E. |
author_facet | Hasson, Dan Panchenko, Tanya Salimian, Kevan J. Salman, Mishah U. Sekulic, Nikolina Alonso, Alicia Warburton, Peter E. Black, Ben E. |
author_sort | Hasson, Dan |
collection | PubMed |
description | The centromere is the chromosomal locus that ensures fidelity in genome transmission at cell division. Centromere protein A (CENP-A) is a histone H3 variant that specifies centromere location independently of DNA sequence. Conflicting evidence has emerged regarding the histone composition and stoichiometry of CENP-A nucleosomes. Here we show that the predominant form of the CENP-A particle at human centromeres is an octameric nucleosome. CENP-A nucleosomes are very highly phased on α-satellite 171 bp monomers at normal centromeres, and also display strong positioning at neocentromeres. At either type of functional centromere, CENP-A nucleosomes exhibit similar DNA wrapping behavior as octameric CENP-A nucleosomes reconstituted with recombinant components, having looser DNA termini than those on their conventional counterparts containing canonical H3. Thus, the fundamental unit of the chromatin that epigenetically specifies centromere location in mammals is an octameric nucleosome with loose termini. |
format | Online Article Text |
id | pubmed-3760417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-37604172013-12-01 The octamer is the major form of CENP-A nucleosomes at human centromeres Hasson, Dan Panchenko, Tanya Salimian, Kevan J. Salman, Mishah U. Sekulic, Nikolina Alonso, Alicia Warburton, Peter E. Black, Ben E. Nat Struct Mol Biol Article The centromere is the chromosomal locus that ensures fidelity in genome transmission at cell division. Centromere protein A (CENP-A) is a histone H3 variant that specifies centromere location independently of DNA sequence. Conflicting evidence has emerged regarding the histone composition and stoichiometry of CENP-A nucleosomes. Here we show that the predominant form of the CENP-A particle at human centromeres is an octameric nucleosome. CENP-A nucleosomes are very highly phased on α-satellite 171 bp monomers at normal centromeres, and also display strong positioning at neocentromeres. At either type of functional centromere, CENP-A nucleosomes exhibit similar DNA wrapping behavior as octameric CENP-A nucleosomes reconstituted with recombinant components, having looser DNA termini than those on their conventional counterparts containing canonical H3. Thus, the fundamental unit of the chromatin that epigenetically specifies centromere location in mammals is an octameric nucleosome with loose termini. 2013-05-05 2013-06 /pmc/articles/PMC3760417/ /pubmed/23644596 http://dx.doi.org/10.1038/nsmb.2562 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Hasson, Dan Panchenko, Tanya Salimian, Kevan J. Salman, Mishah U. Sekulic, Nikolina Alonso, Alicia Warburton, Peter E. Black, Ben E. The octamer is the major form of CENP-A nucleosomes at human centromeres |
title | The octamer is the major form of CENP-A nucleosomes at human centromeres |
title_full | The octamer is the major form of CENP-A nucleosomes at human centromeres |
title_fullStr | The octamer is the major form of CENP-A nucleosomes at human centromeres |
title_full_unstemmed | The octamer is the major form of CENP-A nucleosomes at human centromeres |
title_short | The octamer is the major form of CENP-A nucleosomes at human centromeres |
title_sort | octamer is the major form of cenp-a nucleosomes at human centromeres |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760417/ https://www.ncbi.nlm.nih.gov/pubmed/23644596 http://dx.doi.org/10.1038/nsmb.2562 |
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