Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis

Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration o...

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Detalles Bibliográficos
Autores principales: Gardiner, Chris, Ferreira, Yannick J., Dragovic, Rebecca A., Redman, Christopher W.G., Sargent, Ian L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Co-Action Publishing 2013
Materias:
Original Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760643/
https://www.ncbi.nlm.nih.gov/pubmed/24009893
http://dx.doi.org/10.3402/jev.v2i0.19671
Descripción
Sumario:Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.

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