Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis

Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration o...

Descripción completa

Detalles Bibliográficos
Autores principales: Gardiner, Chris, Ferreira, Yannick J., Dragovic, Rebecca A., Redman, Christopher W.G., Sargent, Ian L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Co-Action Publishing 2013
Materias:
Original Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760643/
https://www.ncbi.nlm.nih.gov/pubmed/24009893
http://dx.doi.org/10.3402/jev.v2i0.19671
_version_ 1782282778431193088
author Gardiner, Chris
Ferreira, Yannick J.
Dragovic, Rebecca A.
Redman, Christopher W.G.
Sargent, Ian L.
author_facet Gardiner, Chris
Ferreira, Yannick J.
Dragovic, Rebecca A.
Redman, Christopher W.G.
Sargent, Ian L.
author_sort Gardiner, Chris
collection PubMed
description Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.
format Online
Article
Text
id pubmed-3760643
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Co-Action Publishing
record_format MEDLINE/PubMed
spelling pubmed-37606432013-09-04 Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis Gardiner, Chris Ferreira, Yannick J. Dragovic, Rebecca A. Redman, Christopher W.G. Sargent, Ian L. J Extracell Vesicles Original Research Article Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique. Co-Action Publishing 2013-02-15 /pmc/articles/PMC3760643/ /pubmed/24009893 http://dx.doi.org/10.3402/jev.v2i0.19671 Text en © 2013 Chris Gardiner et al. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Article
Gardiner, Chris
Ferreira, Yannick J.
Dragovic, Rebecca A.
Redman, Christopher W.G.
Sargent, Ian L.
Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis
title Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis
title_full Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis
title_fullStr Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis
title_full_unstemmed Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis
title_short Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis
title_sort extracellular vesicle sizing and enumeration by nanoparticle tracking analysis
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760643/
https://www.ncbi.nlm.nih.gov/pubmed/24009893
http://dx.doi.org/10.3402/jev.v2i0.19671
work_keys_str_mv AT gardinerchris extracellularvesiclesizingandenumerationbynanoparticletrackinganalysis
AT ferreirayannickj extracellularvesiclesizingandenumerationbynanoparticletrackinganalysis
AT dragovicrebeccaa extracellularvesiclesizingandenumerationbynanoparticletrackinganalysis
AT redmanchristopherwg extracellularvesiclesizingandenumerationbynanoparticletrackinganalysis
AT sargentianl extracellularvesiclesizingandenumerationbynanoparticletrackinganalysis

Ejemplares similares