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TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica

Our gastrointestinal tract is a portal of entry for a number of bacteria and viruses. Thus, this tissue must develop ways to induce antigen-specific T cell and antibody responses quickly. Intestinal epithelial cells are a central player in barrier function and also in communicating signals from inva...

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Autores principales: Sugiura, Y, Kamdar, K, Khakpour, S, Young, G, Karpus, W J, DePaolo, R William
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760963/
https://www.ncbi.nlm.nih.gov/pubmed/23443468
http://dx.doi.org/10.1038/mi.2013.5
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author Sugiura, Y
Kamdar, K
Khakpour, S
Young, G
Karpus, W J
DePaolo, R William
author_facet Sugiura, Y
Kamdar, K
Khakpour, S
Young, G
Karpus, W J
DePaolo, R William
author_sort Sugiura, Y
collection PubMed
description Our gastrointestinal tract is a portal of entry for a number of bacteria and viruses. Thus, this tissue must develop ways to induce antigen-specific T cell and antibody responses quickly. Intestinal epithelial cells are a central player in barrier function and also in communicating signals from invading pathogens to the underlying immune tissue. Here we demonstrate that activation of Toll-like receptor 1 (TLR1) in the epithelium leads to the upregulation of the chemokine CCL20 during oral infection with Yersinia enterocolitica. Further, both neutralization of CCL20 using polyclonal antibody treatment and deletion of TLR1 resulted in a defect in CCR6+ dendritic cells (DCs), which produce innate cytokines that help to induce anti-Yersinia-specific T helper 17 (T(H)17) cells and IgA production. These data demonstrate a novel role for TLR1 signaling in the intestinal epithelium and demonstrate that together TLR1 and CCL20 are critical mediators of T(H)17 immunity through the activation and recruitment of DCs.
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spelling pubmed-37609632013-10-23 TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica Sugiura, Y Kamdar, K Khakpour, S Young, G Karpus, W J DePaolo, R William Mucosal Immunol Article Our gastrointestinal tract is a portal of entry for a number of bacteria and viruses. Thus, this tissue must develop ways to induce antigen-specific T cell and antibody responses quickly. Intestinal epithelial cells are a central player in barrier function and also in communicating signals from invading pathogens to the underlying immune tissue. Here we demonstrate that activation of Toll-like receptor 1 (TLR1) in the epithelium leads to the upregulation of the chemokine CCL20 during oral infection with Yersinia enterocolitica. Further, both neutralization of CCL20 using polyclonal antibody treatment and deletion of TLR1 resulted in a defect in CCR6+ dendritic cells (DCs), which produce innate cytokines that help to induce anti-Yersinia-specific T helper 17 (T(H)17) cells and IgA production. These data demonstrate a novel role for TLR1 signaling in the intestinal epithelium and demonstrate that together TLR1 and CCL20 are critical mediators of T(H)17 immunity through the activation and recruitment of DCs. Nature Publishing Group 2013-11 2013-02-27 /pmc/articles/PMC3760963/ /pubmed/23443468 http://dx.doi.org/10.1038/mi.2013.5 Text en Copyright © 2013 Society for Mucosal Immunology http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Sugiura, Y
Kamdar, K
Khakpour, S
Young, G
Karpus, W J
DePaolo, R William
TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica
title TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica
title_full TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica
title_fullStr TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica
title_full_unstemmed TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica
title_short TLR1-induced chemokine production is critical for mucosal immunity against Yersinia enterocolitica
title_sort tlr1-induced chemokine production is critical for mucosal immunity against yersinia enterocolitica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760963/
https://www.ncbi.nlm.nih.gov/pubmed/23443468
http://dx.doi.org/10.1038/mi.2013.5
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