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Development of the Schedule for Multiple Parallel “Difficult” Peptide Synthesis on Pins
Unified schedule for multiple parallel solid-phase synthesis of so-called “difficult” peptides on polypropylene pins was developed. Increase in the efficiency of 9-fluorenyl(methoxycarbonyl) N-terminal amino-protecting group removal was shown to have a greater influence on the accuracy of the “diffi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3762146/ https://www.ncbi.nlm.nih.gov/pubmed/24027588 http://dx.doi.org/10.1155/2013/197317 |
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author | Kolesanova, Ekaterina F. Sanzhakov, Maxim A. Kharybin, Oleg N. |
author_facet | Kolesanova, Ekaterina F. Sanzhakov, Maxim A. Kharybin, Oleg N. |
author_sort | Kolesanova, Ekaterina F. |
collection | PubMed |
description | Unified schedule for multiple parallel solid-phase synthesis of so-called “difficult” peptides on polypropylene pins was developed. Increase in the efficiency of 9-fluorenyl(methoxycarbonyl) N-terminal amino-protecting group removal was shown to have a greater influence on the accuracy of the “difficult” peptide synthesis than the use of more efficient amino acid coupling reagents such as aminium salts. Hence the unified schedule for multiple parallel solid-phase synthesis of “difficult” peptides included the procedure for N-terminal amino group deprotection modified by applying a more efficient reagent for the deprotection and the standard procedure of amino acid coupling by carbodiimide method with an additional coupling using aminium salts, if necessary. Amino acid coupling with the help of carbodiimide allows to follow the completeness of the coupling via the bromophenol blue indication, thus providing the accuracy of the synthesis and preventing an overexpenditure of expensive reagents. About 100 biotinylated hepatitis C virus envelope protein fragments, most of which represented “difficult” peptides, were successfully obtained by synthesis on pins with the help of the developed unified schedule. |
format | Online Article Text |
id | pubmed-3762146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-37621462013-09-11 Development of the Schedule for Multiple Parallel “Difficult” Peptide Synthesis on Pins Kolesanova, Ekaterina F. Sanzhakov, Maxim A. Kharybin, Oleg N. Int J Pept Research Article Unified schedule for multiple parallel solid-phase synthesis of so-called “difficult” peptides on polypropylene pins was developed. Increase in the efficiency of 9-fluorenyl(methoxycarbonyl) N-terminal amino-protecting group removal was shown to have a greater influence on the accuracy of the “difficult” peptide synthesis than the use of more efficient amino acid coupling reagents such as aminium salts. Hence the unified schedule for multiple parallel solid-phase synthesis of “difficult” peptides included the procedure for N-terminal amino group deprotection modified by applying a more efficient reagent for the deprotection and the standard procedure of amino acid coupling by carbodiimide method with an additional coupling using aminium salts, if necessary. Amino acid coupling with the help of carbodiimide allows to follow the completeness of the coupling via the bromophenol blue indication, thus providing the accuracy of the synthesis and preventing an overexpenditure of expensive reagents. About 100 biotinylated hepatitis C virus envelope protein fragments, most of which represented “difficult” peptides, were successfully obtained by synthesis on pins with the help of the developed unified schedule. Hindawi Publishing Corporation 2013 2013-08-20 /pmc/articles/PMC3762146/ /pubmed/24027588 http://dx.doi.org/10.1155/2013/197317 Text en Copyright © 2013 Ekaterina F. Kolesanova et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kolesanova, Ekaterina F. Sanzhakov, Maxim A. Kharybin, Oleg N. Development of the Schedule for Multiple Parallel “Difficult” Peptide Synthesis on Pins |
title | Development of the Schedule for Multiple Parallel “Difficult” Peptide Synthesis on Pins |
title_full | Development of the Schedule for Multiple Parallel “Difficult” Peptide Synthesis on Pins |
title_fullStr | Development of the Schedule for Multiple Parallel “Difficult” Peptide Synthesis on Pins |
title_full_unstemmed | Development of the Schedule for Multiple Parallel “Difficult” Peptide Synthesis on Pins |
title_short | Development of the Schedule for Multiple Parallel “Difficult” Peptide Synthesis on Pins |
title_sort | development of the schedule for multiple parallel “difficult” peptide synthesis on pins |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3762146/ https://www.ncbi.nlm.nih.gov/pubmed/24027588 http://dx.doi.org/10.1155/2013/197317 |
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