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Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species

Cells are fundamental units of life, but little is known about evolution of cell states. Induced pluripotent stem cells (iPSCs) are once differentiated cells that have been re-programmed to an embryonic stem cell-like state, providing a powerful platform for biology and medicine. However, they have...

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Autores principales: Rosselló, Ricardo Antonio, Chen, Chun-Chun, Dai, Rui, Howard, Jason T, Hochgeschwender, Ute, Jarvis, Erich D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3762186/
https://www.ncbi.nlm.nih.gov/pubmed/24015354
http://dx.doi.org/10.7554/eLife.00036
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author Rosselló, Ricardo Antonio
Chen, Chun-Chun
Dai, Rui
Howard, Jason T
Hochgeschwender, Ute
Jarvis, Erich D
author_facet Rosselló, Ricardo Antonio
Chen, Chun-Chun
Dai, Rui
Howard, Jason T
Hochgeschwender, Ute
Jarvis, Erich D
author_sort Rosselló, Ricardo Antonio
collection PubMed
description Cells are fundamental units of life, but little is known about evolution of cell states. Induced pluripotent stem cells (iPSCs) are once differentiated cells that have been re-programmed to an embryonic stem cell-like state, providing a powerful platform for biology and medicine. However, they have been limited to a few mammalian species. Here we found that a set of four mammalian transcription factor genes used to generate iPSCs in mouse and humans can induce a partially reprogrammed pluripotent stem cell (PRPSCs) state in vertebrate and invertebrate model organisms, in mammals, birds, fish, and fly, which span 550 million years from a common ancestor. These findings are one of the first to show cross-lineage stem cell-like induction, and to generate pluripotent-like cells for several of these species with in vivo chimeras. We suggest that the stem-cell state may be highly conserved across a wide phylogenetic range. DOI: http://dx.doi.org/10.7554/eLife.00036.001
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spelling pubmed-37621862013-09-06 Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species Rosselló, Ricardo Antonio Chen, Chun-Chun Dai, Rui Howard, Jason T Hochgeschwender, Ute Jarvis, Erich D eLife Developmental Biology and Stem Cells Cells are fundamental units of life, but little is known about evolution of cell states. Induced pluripotent stem cells (iPSCs) are once differentiated cells that have been re-programmed to an embryonic stem cell-like state, providing a powerful platform for biology and medicine. However, they have been limited to a few mammalian species. Here we found that a set of four mammalian transcription factor genes used to generate iPSCs in mouse and humans can induce a partially reprogrammed pluripotent stem cell (PRPSCs) state in vertebrate and invertebrate model organisms, in mammals, birds, fish, and fly, which span 550 million years from a common ancestor. These findings are one of the first to show cross-lineage stem cell-like induction, and to generate pluripotent-like cells for several of these species with in vivo chimeras. We suggest that the stem-cell state may be highly conserved across a wide phylogenetic range. DOI: http://dx.doi.org/10.7554/eLife.00036.001 eLife Sciences Publications, Ltd 2013-09-03 /pmc/articles/PMC3762186/ /pubmed/24015354 http://dx.doi.org/10.7554/eLife.00036 Text en Copyright © 2013, Rosselló et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology and Stem Cells
Rosselló, Ricardo Antonio
Chen, Chun-Chun
Dai, Rui
Howard, Jason T
Hochgeschwender, Ute
Jarvis, Erich D
Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species
title Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species
title_full Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species
title_fullStr Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species
title_full_unstemmed Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species
title_short Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species
title_sort mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species
topic Developmental Biology and Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3762186/
https://www.ncbi.nlm.nih.gov/pubmed/24015354
http://dx.doi.org/10.7554/eLife.00036
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