Endothelin Receptor Overexpression Alters Diastolic Function in Cultured Rat Ventricular Myocytes

The endothelin (ET) signaling pathway controls many physiological processes in myocardium and often becomes upregulated in heart diseases. The aim of the present study was to investigate the effects of ET receptor upregulation on the contractile function of adult ventricular myocytes. Primary cultured adult rat ventricular myocytes were used as a model system of ET receptor overexpression in the heart. Endothelin receptor type A (ET(A)) or type B (ET(B)) was overexpressed by Adenoviral infection, and the twitch responses of infected ventricular myocytes were measured after ET-1 stimulation. Overexpression of ET(A) exaggerated positive inotropic effect (PIE) and diastolic shortening of ET-1, and induced a new twitch response including twitch broadening. On the contrary, overexpression of ET(B) increased PIE of ET-1, but did not affect other two twitch responses. Control myocytes expressing endogenous receptors showed a parallel increase in twitch amplitude and systolic Ca(2+) in response to ET-1. However, intracellular Ca(2+) did not change in proportion to the changes in contractility in myocytes overexpressing ET(A). Overexpression of ET(A) enhanced both systolic and diastolic contractility without parallel changes in Ca(2+). Differential regulation of this nature indicates that upregulation of ET(A) may contribute to diastolic myocardial dysfunction by selectively targeting myofi lament proteins that regulate resting cell length, twitch duration and responsiveness to prevailing Ca(2+).