Dual Role of φ29 DNA Polymerase Lys529 in Stabilisation of the DNA Priming-Terminus and the Terminal Protein-Priming Residue at the Polymerisation Site

Resolution of the crystallographic structure of φ29 DNA polymerase binary and ternary complexes showed that residue Lys529, located at the C-terminus of the palm subdomain, establishes contacts with the 3′ terminal phosphodiester bond. In this paper, site-directed mutants at this Lys residue were us...

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Detalles Bibliográficos
Autores principales: del Prado, Alicia, Lázaro, José M., Villar, Laurentino, Salas, Margarita, de Vega, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3762793/
https://www.ncbi.nlm.nih.gov/pubmed/24023769
http://dx.doi.org/10.1371/journal.pone.0072765

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