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Gene-Based Antibody Strategies for Prion Diseases
Prion diseases or transmissible spongiform encephalopathies (TSE) are a group of neurodegenerative and infectious disorders characterized by the conversion of a normal cellular protein PrP(C) into a pathological abnormally folded form, termed PrP(Sc). There are neither available therapies nor diagno...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763265/ https://www.ncbi.nlm.nih.gov/pubmed/24027584 http://dx.doi.org/10.1155/2013/710406 |
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author | Cardinale, Alessio Biocca, Silvia |
author_facet | Cardinale, Alessio Biocca, Silvia |
author_sort | Cardinale, Alessio |
collection | PubMed |
description | Prion diseases or transmissible spongiform encephalopathies (TSE) are a group of neurodegenerative and infectious disorders characterized by the conversion of a normal cellular protein PrP(C) into a pathological abnormally folded form, termed PrP(Sc). There are neither available therapies nor diagnostic tools for an early identification of individuals affected by these diseases. New gene-based antibody strategies are emerging as valuable therapeutic tools. Among these, intrabodies are chimeric molecules composed by recombinant antibody fragments fused to intracellular trafficking sequences, aimed at inhibiting, in vivo, the function of specific therapeutic targets. The advantage of intrabodies is that they can be selected against a precise epitope of target proteins, including protein-protein interaction sites and cytotoxic conformers (i.e., oligomeric and fibrillar assemblies). Herein, we address and discuss in vitro and in vivo applications of intrabodies in prion diseases, focussing on their therapeutic potential. |
format | Online Article Text |
id | pubmed-3763265 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-37632652013-09-11 Gene-Based Antibody Strategies for Prion Diseases Cardinale, Alessio Biocca, Silvia Int J Cell Biol Review Article Prion diseases or transmissible spongiform encephalopathies (TSE) are a group of neurodegenerative and infectious disorders characterized by the conversion of a normal cellular protein PrP(C) into a pathological abnormally folded form, termed PrP(Sc). There are neither available therapies nor diagnostic tools for an early identification of individuals affected by these diseases. New gene-based antibody strategies are emerging as valuable therapeutic tools. Among these, intrabodies are chimeric molecules composed by recombinant antibody fragments fused to intracellular trafficking sequences, aimed at inhibiting, in vivo, the function of specific therapeutic targets. The advantage of intrabodies is that they can be selected against a precise epitope of target proteins, including protein-protein interaction sites and cytotoxic conformers (i.e., oligomeric and fibrillar assemblies). Herein, we address and discuss in vitro and in vivo applications of intrabodies in prion diseases, focussing on their therapeutic potential. Hindawi Publishing Corporation 2013 2013-08-21 /pmc/articles/PMC3763265/ /pubmed/24027584 http://dx.doi.org/10.1155/2013/710406 Text en Copyright © 2013 A. Cardinale and S. Biocca. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Cardinale, Alessio Biocca, Silvia Gene-Based Antibody Strategies for Prion Diseases |
title | Gene-Based Antibody Strategies for Prion Diseases |
title_full | Gene-Based Antibody Strategies for Prion Diseases |
title_fullStr | Gene-Based Antibody Strategies for Prion Diseases |
title_full_unstemmed | Gene-Based Antibody Strategies for Prion Diseases |
title_short | Gene-Based Antibody Strategies for Prion Diseases |
title_sort | gene-based antibody strategies for prion diseases |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763265/ https://www.ncbi.nlm.nih.gov/pubmed/24027584 http://dx.doi.org/10.1155/2013/710406 |
work_keys_str_mv | AT cardinalealessio genebasedantibodystrategiesforpriondiseases AT bioccasilvia genebasedantibodystrategiesforpriondiseases |