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Discriminating Different Cancer Cells Using a Zebrafish in Vivo Assay

Despite the expanded understanding of tumor angiogenesis phenomenon and how it impacts cancer treatment outcomes, we have yet to develop a robust assay that can quickly, easily, and quantitatively measure tumor-induced angiogenesis. Since the zebrafish/tumor xenograft represents an emerging tool in...

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Autores principales: Moshal, Karni S., Ferri-Lagneau, Karine F., Haider, Jamil, Pardhanani, Pooja, Leung, TinChung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763412/
https://www.ncbi.nlm.nih.gov/pubmed/24213127
http://dx.doi.org/10.3390/cancers3044102
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author Moshal, Karni S.
Ferri-Lagneau, Karine F.
Haider, Jamil
Pardhanani, Pooja
Leung, TinChung
author_facet Moshal, Karni S.
Ferri-Lagneau, Karine F.
Haider, Jamil
Pardhanani, Pooja
Leung, TinChung
author_sort Moshal, Karni S.
collection PubMed
description Despite the expanded understanding of tumor angiogenesis phenomenon and how it impacts cancer treatment outcomes, we have yet to develop a robust assay that can quickly, easily, and quantitatively measure tumor-induced angiogenesis. Since the zebrafish/tumor xenograft represents an emerging tool in this regard, the present study strives to capitalize on the ease, effectiveness, and the adaptability of this model to quantify tumor angiogenesis. In order to test a range of responses, we chose two different tumorigenic cell lines, the human non-small cell lung carcinoma (H1299) and the mouse lung adenocarcinoma (CL13). Non-tumorigenic 3T3-L1 cells served as negative control. The cells were grafted near to the perivitelline space of the zebrafish embryos and the angiogenic response was analyzed using whole-mount alkaline phosphatase (AP) vessel staining and fluorescence microscopy. Angiogenic activity was scored based on the length and number of the newly formed ectopic vessels and the percentage of embryos with ectopic vessels. At 2 day-post-implantation, we detected a significant increase in the length and number of ectopic vessels with H1299 cell implantation compared to CL13 cell transplantation, both are higher than 3T3-L1 control. We also observed a significantly higher percentage of embryos with ectopic vessels with H1299 and CL13 transplantation compared to the 3T3-L1 control, but this parameter is not as robust and reliable as measuring the length and number of ectopic vessels. Furthermore, the systemic exposure of zebrafish embryos to an anti-angiogenesis drug (PTK 787, inhibitor of vascular endothelial growth factor receptor tyrosine kinase) inhibited tumor-induced angiogenesis, suggesting that the assay can be used to evaluate anti-angiogenic drugs. This study implicates the feasibility of using zebrafish xenotransplantation to perform quantitative measurement of the angiogenic activity of cancer cells which can be further extended to measure cancer cell metastasis. This assay represents not only the useful test for patient diagnosis, but also has the potential for evaluating anti-cancer drugs treatment.
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spelling pubmed-37634122013-09-05 Discriminating Different Cancer Cells Using a Zebrafish in Vivo Assay Moshal, Karni S. Ferri-Lagneau, Karine F. Haider, Jamil Pardhanani, Pooja Leung, TinChung Cancers (Basel) Article Despite the expanded understanding of tumor angiogenesis phenomenon and how it impacts cancer treatment outcomes, we have yet to develop a robust assay that can quickly, easily, and quantitatively measure tumor-induced angiogenesis. Since the zebrafish/tumor xenograft represents an emerging tool in this regard, the present study strives to capitalize on the ease, effectiveness, and the adaptability of this model to quantify tumor angiogenesis. In order to test a range of responses, we chose two different tumorigenic cell lines, the human non-small cell lung carcinoma (H1299) and the mouse lung adenocarcinoma (CL13). Non-tumorigenic 3T3-L1 cells served as negative control. The cells were grafted near to the perivitelline space of the zebrafish embryos and the angiogenic response was analyzed using whole-mount alkaline phosphatase (AP) vessel staining and fluorescence microscopy. Angiogenic activity was scored based on the length and number of the newly formed ectopic vessels and the percentage of embryos with ectopic vessels. At 2 day-post-implantation, we detected a significant increase in the length and number of ectopic vessels with H1299 cell implantation compared to CL13 cell transplantation, both are higher than 3T3-L1 control. We also observed a significantly higher percentage of embryos with ectopic vessels with H1299 and CL13 transplantation compared to the 3T3-L1 control, but this parameter is not as robust and reliable as measuring the length and number of ectopic vessels. Furthermore, the systemic exposure of zebrafish embryos to an anti-angiogenesis drug (PTK 787, inhibitor of vascular endothelial growth factor receptor tyrosine kinase) inhibited tumor-induced angiogenesis, suggesting that the assay can be used to evaluate anti-angiogenic drugs. This study implicates the feasibility of using zebrafish xenotransplantation to perform quantitative measurement of the angiogenic activity of cancer cells which can be further extended to measure cancer cell metastasis. This assay represents not only the useful test for patient diagnosis, but also has the potential for evaluating anti-cancer drugs treatment. Molecular Diversity Preservation International (MDPI) 2011-10-31 /pmc/articles/PMC3763412/ /pubmed/24213127 http://dx.doi.org/10.3390/cancers3044102 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Moshal, Karni S.
Ferri-Lagneau, Karine F.
Haider, Jamil
Pardhanani, Pooja
Leung, TinChung
Discriminating Different Cancer Cells Using a Zebrafish in Vivo Assay
title Discriminating Different Cancer Cells Using a Zebrafish in Vivo Assay
title_full Discriminating Different Cancer Cells Using a Zebrafish in Vivo Assay
title_fullStr Discriminating Different Cancer Cells Using a Zebrafish in Vivo Assay
title_full_unstemmed Discriminating Different Cancer Cells Using a Zebrafish in Vivo Assay
title_short Discriminating Different Cancer Cells Using a Zebrafish in Vivo Assay
title_sort discriminating different cancer cells using a zebrafish in vivo assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763412/
https://www.ncbi.nlm.nih.gov/pubmed/24213127
http://dx.doi.org/10.3390/cancers3044102
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