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Probiotic Ferulic Acid Esterase Active Lactobacillus fermentum NCIMB 5221 APA Microcapsules for Oral Delivery: Preparation and in Vitro Characterization
Probiotics possess potential therapeutic and preventative effects for various diseases and metabolic disorders. One important limitation for the oral delivery of probiotics is the harsh conditions of the upper gastrointestinal tract (GIT) which challenge bacterial viability and activity. One propose...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763630/ https://www.ncbi.nlm.nih.gov/pubmed/24288090 http://dx.doi.org/10.3390/ph5020236 |
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author | Tomaro-Duchesneau, Catherine Saha, Shyamali Malhotra, Meenakshi Coussa-Charley, Michael Kahouli, Imen Jones, Mitchell L. Labbé, Alain Prakash, Satya |
author_facet | Tomaro-Duchesneau, Catherine Saha, Shyamali Malhotra, Meenakshi Coussa-Charley, Michael Kahouli, Imen Jones, Mitchell L. Labbé, Alain Prakash, Satya |
author_sort | Tomaro-Duchesneau, Catherine |
collection | PubMed |
description | Probiotics possess potential therapeutic and preventative effects for various diseases and metabolic disorders. One important limitation for the oral delivery of probiotics is the harsh conditions of the upper gastrointestinal tract (GIT) which challenge bacterial viability and activity. One proposed method to surpass this obstacle is the use of microencapsulation to improve the delivery of bacterial cells to the lower GIT. The aim of this study is to use alginate-poly-L-lysine-alginate (APA) microcapsules to encapsulate Lactobacillus fermentum NCIMB 5221 and characterize its enzymatic activity and viability through a simulated GIT. This specific strain, in previous research, was characterized for its inherent ferulic acid esterase (FAE) activity which could prove beneficial in the development of a therapeutic for the treatment and prevention of cancers and metabolic disorders. Our findings demonstrate that the APA microcapsule does not slow the mass transfer of substrate into and that of the FA product out of the microcapsule, while also not impairing bacterial cell viability. The use of simulated gastrointestinal conditions led to a significant 2.5 log difference in viability between the free (1.10 × 10(4) ± 1.00 × 10(3) cfu/mL) and the microencapsulated (5.50 × 10(6) ± 1.00 × 10(5) cfu/mL) L. fermentum NCIMB 5221 following exposure. The work presented here suggests that APA microencapsulation can be used as an effective oral delivery method for L. fermentum NCIMB 5221, a FAE-active probiotic strain. |
format | Online Article Text |
id | pubmed-3763630 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-37636302013-11-14 Probiotic Ferulic Acid Esterase Active Lactobacillus fermentum NCIMB 5221 APA Microcapsules for Oral Delivery: Preparation and in Vitro Characterization Tomaro-Duchesneau, Catherine Saha, Shyamali Malhotra, Meenakshi Coussa-Charley, Michael Kahouli, Imen Jones, Mitchell L. Labbé, Alain Prakash, Satya Pharmaceuticals (Basel) Article Probiotics possess potential therapeutic and preventative effects for various diseases and metabolic disorders. One important limitation for the oral delivery of probiotics is the harsh conditions of the upper gastrointestinal tract (GIT) which challenge bacterial viability and activity. One proposed method to surpass this obstacle is the use of microencapsulation to improve the delivery of bacterial cells to the lower GIT. The aim of this study is to use alginate-poly-L-lysine-alginate (APA) microcapsules to encapsulate Lactobacillus fermentum NCIMB 5221 and characterize its enzymatic activity and viability through a simulated GIT. This specific strain, in previous research, was characterized for its inherent ferulic acid esterase (FAE) activity which could prove beneficial in the development of a therapeutic for the treatment and prevention of cancers and metabolic disorders. Our findings demonstrate that the APA microcapsule does not slow the mass transfer of substrate into and that of the FA product out of the microcapsule, while also not impairing bacterial cell viability. The use of simulated gastrointestinal conditions led to a significant 2.5 log difference in viability between the free (1.10 × 10(4) ± 1.00 × 10(3) cfu/mL) and the microencapsulated (5.50 × 10(6) ± 1.00 × 10(5) cfu/mL) L. fermentum NCIMB 5221 following exposure. The work presented here suggests that APA microencapsulation can be used as an effective oral delivery method for L. fermentum NCIMB 5221, a FAE-active probiotic strain. MDPI 2012-02-16 /pmc/articles/PMC3763630/ /pubmed/24288090 http://dx.doi.org/10.3390/ph5020236 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Tomaro-Duchesneau, Catherine Saha, Shyamali Malhotra, Meenakshi Coussa-Charley, Michael Kahouli, Imen Jones, Mitchell L. Labbé, Alain Prakash, Satya Probiotic Ferulic Acid Esterase Active Lactobacillus fermentum NCIMB 5221 APA Microcapsules for Oral Delivery: Preparation and in Vitro Characterization |
title | Probiotic Ferulic Acid Esterase Active Lactobacillus fermentum NCIMB 5221 APA Microcapsules for Oral Delivery: Preparation and in Vitro Characterization |
title_full | Probiotic Ferulic Acid Esterase Active Lactobacillus fermentum NCIMB 5221 APA Microcapsules for Oral Delivery: Preparation and in Vitro Characterization |
title_fullStr | Probiotic Ferulic Acid Esterase Active Lactobacillus fermentum NCIMB 5221 APA Microcapsules for Oral Delivery: Preparation and in Vitro Characterization |
title_full_unstemmed | Probiotic Ferulic Acid Esterase Active Lactobacillus fermentum NCIMB 5221 APA Microcapsules for Oral Delivery: Preparation and in Vitro Characterization |
title_short | Probiotic Ferulic Acid Esterase Active Lactobacillus fermentum NCIMB 5221 APA Microcapsules for Oral Delivery: Preparation and in Vitro Characterization |
title_sort | probiotic ferulic acid esterase active lactobacillus fermentum ncimb 5221 apa microcapsules for oral delivery: preparation and in vitro characterization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763630/ https://www.ncbi.nlm.nih.gov/pubmed/24288090 http://dx.doi.org/10.3390/ph5020236 |
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