Cargando…

Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines

Scrophularia ningpoensis has been used in China for centuries as a herbal tea to treat various diseases. Based on the numerous animal studies on its pharmaceutical effects and the long time clinical experiences, we studied the molecular and cellular mechanism underlying the bioactivity of aqueous ex...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Xiao, Eichhorn, Tolga, Greten, Henry Johannes, Efferth, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763631/
https://www.ncbi.nlm.nih.gov/pubmed/24288088
http://dx.doi.org/10.3390/ph5020189
_version_ 1782283048923955200
author Shen, Xiao
Eichhorn, Tolga
Greten, Henry Johannes
Efferth, Thomas
author_facet Shen, Xiao
Eichhorn, Tolga
Greten, Henry Johannes
Efferth, Thomas
author_sort Shen, Xiao
collection PubMed
description Scrophularia ningpoensis has been used in China for centuries as a herbal tea to treat various diseases. Based on the numerous animal studies on its pharmaceutical effects and the long time clinical experiences, we studied the molecular and cellular mechanism underlying the bioactivity of aqueous extract of Scrophularia and its isolated compounds. Seven isolated compounds, unlike Scrophularia extract, failed to induce cytotoxicity on HaCaT cells, but their combination improved the effect of extract. Tumor cell line selectivity was not observed, when we studied its cytotoxic effect on melanoma cell lines. The apoptotic and anti-inflammatory effects of Scrophularia extract have been demonstrated on HaCaT cells. The extract induced those effects potentially through affecting the MAPK pathway and inhibition of the NF-κB pathway, Microarray-based bioinformatical analyses on the compound acetoside from Scrophularia revealed a gene expression profile which confirmed our findings with the extract on proliferation inhibition, anti-inflammation and apoptosis. With DNA alkylation as major proposed mechanism of action, we assume acetoside as one of the active compounds in Scrophularia.
format Online
Article
Text
id pubmed-3763631
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-37636312013-11-14 Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines Shen, Xiao Eichhorn, Tolga Greten, Henry Johannes Efferth, Thomas Pharmaceuticals (Basel) Article Scrophularia ningpoensis has been used in China for centuries as a herbal tea to treat various diseases. Based on the numerous animal studies on its pharmaceutical effects and the long time clinical experiences, we studied the molecular and cellular mechanism underlying the bioactivity of aqueous extract of Scrophularia and its isolated compounds. Seven isolated compounds, unlike Scrophularia extract, failed to induce cytotoxicity on HaCaT cells, but their combination improved the effect of extract. Tumor cell line selectivity was not observed, when we studied its cytotoxic effect on melanoma cell lines. The apoptotic and anti-inflammatory effects of Scrophularia extract have been demonstrated on HaCaT cells. The extract induced those effects potentially through affecting the MAPK pathway and inhibition of the NF-κB pathway, Microarray-based bioinformatical analyses on the compound acetoside from Scrophularia revealed a gene expression profile which confirmed our findings with the extract on proliferation inhibition, anti-inflammation and apoptosis. With DNA alkylation as major proposed mechanism of action, we assume acetoside as one of the active compounds in Scrophularia. MDPI 2012-02-14 /pmc/articles/PMC3763631/ /pubmed/24288088 http://dx.doi.org/10.3390/ph5020189 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Shen, Xiao
Eichhorn, Tolga
Greten, Henry Johannes
Efferth, Thomas
Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines
title Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines
title_full Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines
title_fullStr Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines
title_full_unstemmed Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines
title_short Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Lines
title_sort effects of scrophularia ningpoensis hemsl. on inhibition of proliferation, apoptosis induction and nf-κb signaling of immortalized and cancer cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763631/
https://www.ncbi.nlm.nih.gov/pubmed/24288088
http://dx.doi.org/10.3390/ph5020189
work_keys_str_mv AT shenxiao effectsofscrophularianingpoensishemsloninhibitionofproliferationapoptosisinductionandnfkbsignalingofimmortalizedandcancercelllines
AT eichhorntolga effectsofscrophularianingpoensishemsloninhibitionofproliferationapoptosisinductionandnfkbsignalingofimmortalizedandcancercelllines
AT gretenhenryjohannes effectsofscrophularianingpoensishemsloninhibitionofproliferationapoptosisinductionandnfkbsignalingofimmortalizedandcancercelllines
AT efferththomas effectsofscrophularianingpoensishemsloninhibitionofproliferationapoptosisinductionandnfkbsignalingofimmortalizedandcancercelllines