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Irreversible Electroporation of Human Primary Uveal Melanoma in Enucleated Eyes

Uveal melanoma (UM) is the most common primary intraocular tumor in adults and is characterized by high rates of metastatic disease. Although brachytherapy is the most common globe-sparing treatment option for small- and medium-sized tumors, the treatment is associated with severe adverse reactions...

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Autores principales: Mandel, Yossi, Laufer, Shlomi, Belkin, Michael, Rubinsky, Boris, Pe'er, Jacob, Frenkel, Shahar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764134/
https://www.ncbi.nlm.nih.gov/pubmed/24039721
http://dx.doi.org/10.1371/journal.pone.0071789
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author Mandel, Yossi
Laufer, Shlomi
Belkin, Michael
Rubinsky, Boris
Pe'er, Jacob
Frenkel, Shahar
author_facet Mandel, Yossi
Laufer, Shlomi
Belkin, Michael
Rubinsky, Boris
Pe'er, Jacob
Frenkel, Shahar
author_sort Mandel, Yossi
collection PubMed
description Uveal melanoma (UM) is the most common primary intraocular tumor in adults and is characterized by high rates of metastatic disease. Although brachytherapy is the most common globe-sparing treatment option for small- and medium-sized tumors, the treatment is associated with severe adverse reactions and does not lead to increased survival rates as compared to enucleation. The use of irreversible electroporation (IRE) for tumor ablation has potential advantages in the treatment of tumors in complex organs such as the eye. Following previous theoretical work, herein we evaluate the use of IRE for uveal tumor ablation in human ex vivo eye model. Enucleated eyes of patients with uveal melanoma were treated with short electric pulses (50–100 µs, 1000–2000 V/cm) using a customized electrode design. Tumor bioimpedance was measured before and after treatment and was followed by histopathological evaluation. We found that IRE caused tumor ablation characterized by cell membrane disruption while sparing the non-cellular sclera. Membrane disruption and loss of cellular capacitance were also associated with significant reduction in total tumor impedance and loss of impedance frequency dependence. The effect was more pronounced near the pulsing electrodes and was dependent on time from treatment to fixation. Future studies should further evaluate the potential of IRE as an alternative method of uveal melanoma treatment.
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spelling pubmed-37641342013-09-13 Irreversible Electroporation of Human Primary Uveal Melanoma in Enucleated Eyes Mandel, Yossi Laufer, Shlomi Belkin, Michael Rubinsky, Boris Pe'er, Jacob Frenkel, Shahar PLoS One Research Article Uveal melanoma (UM) is the most common primary intraocular tumor in adults and is characterized by high rates of metastatic disease. Although brachytherapy is the most common globe-sparing treatment option for small- and medium-sized tumors, the treatment is associated with severe adverse reactions and does not lead to increased survival rates as compared to enucleation. The use of irreversible electroporation (IRE) for tumor ablation has potential advantages in the treatment of tumors in complex organs such as the eye. Following previous theoretical work, herein we evaluate the use of IRE for uveal tumor ablation in human ex vivo eye model. Enucleated eyes of patients with uveal melanoma were treated with short electric pulses (50–100 µs, 1000–2000 V/cm) using a customized electrode design. Tumor bioimpedance was measured before and after treatment and was followed by histopathological evaluation. We found that IRE caused tumor ablation characterized by cell membrane disruption while sparing the non-cellular sclera. Membrane disruption and loss of cellular capacitance were also associated with significant reduction in total tumor impedance and loss of impedance frequency dependence. The effect was more pronounced near the pulsing electrodes and was dependent on time from treatment to fixation. Future studies should further evaluate the potential of IRE as an alternative method of uveal melanoma treatment. Public Library of Science 2013-09-05 /pmc/articles/PMC3764134/ /pubmed/24039721 http://dx.doi.org/10.1371/journal.pone.0071789 Text en © 2013 Mandel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mandel, Yossi
Laufer, Shlomi
Belkin, Michael
Rubinsky, Boris
Pe'er, Jacob
Frenkel, Shahar
Irreversible Electroporation of Human Primary Uveal Melanoma in Enucleated Eyes
title Irreversible Electroporation of Human Primary Uveal Melanoma in Enucleated Eyes
title_full Irreversible Electroporation of Human Primary Uveal Melanoma in Enucleated Eyes
title_fullStr Irreversible Electroporation of Human Primary Uveal Melanoma in Enucleated Eyes
title_full_unstemmed Irreversible Electroporation of Human Primary Uveal Melanoma in Enucleated Eyes
title_short Irreversible Electroporation of Human Primary Uveal Melanoma in Enucleated Eyes
title_sort irreversible electroporation of human primary uveal melanoma in enucleated eyes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764134/
https://www.ncbi.nlm.nih.gov/pubmed/24039721
http://dx.doi.org/10.1371/journal.pone.0071789
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