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In Vitro Infection of Pupae with Israeli Acute Paralysis Virus Suggests Disturbance of Transcriptional Homeostasis in Honey Bees (Apis mellifera)

The ongoing decline of honey bee health worldwide is a serious economic and ecological concern. One major contributor to the decline are pathogens, including several honey bee viruses. However, information is limited on the biology of bee viruses and molecular interactions with their hosts. An exper...

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Autores principales: Boncristiani, Humberto F., Evans, Jay D., Chen, Yanping, Pettis, Jeff, Murphy, Charles, Lopez, Dawn L., Simone-Finstrom, Michael, Strand, Micheline, Tarpy, David R., Rueppell, Olav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764161/
https://www.ncbi.nlm.nih.gov/pubmed/24039938
http://dx.doi.org/10.1371/journal.pone.0073429
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author Boncristiani, Humberto F.
Evans, Jay D.
Chen, Yanping
Pettis, Jeff
Murphy, Charles
Lopez, Dawn L.
Simone-Finstrom, Michael
Strand, Micheline
Tarpy, David R.
Rueppell, Olav
author_facet Boncristiani, Humberto F.
Evans, Jay D.
Chen, Yanping
Pettis, Jeff
Murphy, Charles
Lopez, Dawn L.
Simone-Finstrom, Michael
Strand, Micheline
Tarpy, David R.
Rueppell, Olav
author_sort Boncristiani, Humberto F.
collection PubMed
description The ongoing decline of honey bee health worldwide is a serious economic and ecological concern. One major contributor to the decline are pathogens, including several honey bee viruses. However, information is limited on the biology of bee viruses and molecular interactions with their hosts. An experimental protocol to test these systems was developed, using injections of Israeli Acute Paralysis Virus (IAPV) into honey bee pupae reared ex-situ under laboratory conditions. The infected pupae developed pronounced but variable patterns of disease. Symptoms varied from complete cessation of development with no visual evidence of disease to rapid darkening of a part or the entire body. Considerable differences in IAPV titer dynamics were observed, suggesting significant variation in resistance to IAPV among and possibly within honey bee colonies. Thus, selective breeding for virus resistance should be possible. Gene expression analyses of three separate experiments suggest IAPV disruption of transcriptional homeostasis of several fundamental cellular functions, including an up-regulation of the ribosomal biogenesis pathway. These results provide first insights into the mechanisms of IAPV pathogenicity. They mirror a transcriptional survey of honey bees afflicted with Colony Collapse Disorder and thus support the hypothesis that viruses play a critical role in declining honey bee health.
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spelling pubmed-37641612013-09-13 In Vitro Infection of Pupae with Israeli Acute Paralysis Virus Suggests Disturbance of Transcriptional Homeostasis in Honey Bees (Apis mellifera) Boncristiani, Humberto F. Evans, Jay D. Chen, Yanping Pettis, Jeff Murphy, Charles Lopez, Dawn L. Simone-Finstrom, Michael Strand, Micheline Tarpy, David R. Rueppell, Olav PLoS One Research Article The ongoing decline of honey bee health worldwide is a serious economic and ecological concern. One major contributor to the decline are pathogens, including several honey bee viruses. However, information is limited on the biology of bee viruses and molecular interactions with their hosts. An experimental protocol to test these systems was developed, using injections of Israeli Acute Paralysis Virus (IAPV) into honey bee pupae reared ex-situ under laboratory conditions. The infected pupae developed pronounced but variable patterns of disease. Symptoms varied from complete cessation of development with no visual evidence of disease to rapid darkening of a part or the entire body. Considerable differences in IAPV titer dynamics were observed, suggesting significant variation in resistance to IAPV among and possibly within honey bee colonies. Thus, selective breeding for virus resistance should be possible. Gene expression analyses of three separate experiments suggest IAPV disruption of transcriptional homeostasis of several fundamental cellular functions, including an up-regulation of the ribosomal biogenesis pathway. These results provide first insights into the mechanisms of IAPV pathogenicity. They mirror a transcriptional survey of honey bees afflicted with Colony Collapse Disorder and thus support the hypothesis that viruses play a critical role in declining honey bee health. Public Library of Science 2013-09-05 /pmc/articles/PMC3764161/ /pubmed/24039938 http://dx.doi.org/10.1371/journal.pone.0073429 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Boncristiani, Humberto F.
Evans, Jay D.
Chen, Yanping
Pettis, Jeff
Murphy, Charles
Lopez, Dawn L.
Simone-Finstrom, Michael
Strand, Micheline
Tarpy, David R.
Rueppell, Olav
In Vitro Infection of Pupae with Israeli Acute Paralysis Virus Suggests Disturbance of Transcriptional Homeostasis in Honey Bees (Apis mellifera)
title In Vitro Infection of Pupae with Israeli Acute Paralysis Virus Suggests Disturbance of Transcriptional Homeostasis in Honey Bees (Apis mellifera)
title_full In Vitro Infection of Pupae with Israeli Acute Paralysis Virus Suggests Disturbance of Transcriptional Homeostasis in Honey Bees (Apis mellifera)
title_fullStr In Vitro Infection of Pupae with Israeli Acute Paralysis Virus Suggests Disturbance of Transcriptional Homeostasis in Honey Bees (Apis mellifera)
title_full_unstemmed In Vitro Infection of Pupae with Israeli Acute Paralysis Virus Suggests Disturbance of Transcriptional Homeostasis in Honey Bees (Apis mellifera)
title_short In Vitro Infection of Pupae with Israeli Acute Paralysis Virus Suggests Disturbance of Transcriptional Homeostasis in Honey Bees (Apis mellifera)
title_sort in vitro infection of pupae with israeli acute paralysis virus suggests disturbance of transcriptional homeostasis in honey bees (apis mellifera)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764161/
https://www.ncbi.nlm.nih.gov/pubmed/24039938
http://dx.doi.org/10.1371/journal.pone.0073429
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