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Antispasmodic effects of Prangos ferulacea acetone extract and its main component osthole on ileum contraction
Prangos ferulacea is a plant found in the Mediterranean and Middle-east regions used as carminative, anti-flatulent, emollient and antibacterial herb. It is believed that the coumarins are responsible for some of known effects of Prangos. In this research the relaxant effects of P. ferulacea coumrin...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764677/ https://www.ncbi.nlm.nih.gov/pubmed/24019823 |
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author | Sadraei, H Shokoohinia, Y Sajjadi, S E Mozafari, M |
author_facet | Sadraei, H Shokoohinia, Y Sajjadi, S E Mozafari, M |
author_sort | Sadraei, H |
collection | PubMed |
description | Prangos ferulacea is a plant found in the Mediterranean and Middle-east regions used as carminative, anti-flatulent, emollient and antibacterial herb. It is believed that the coumarins are responsible for some of known effects of Prangos. In this research the relaxant effects of P. ferulacea coumrin rich extract as well as osthole as its main prenylated coumarins were investigated on rat ileum contraction in vitro. Relaxant effect of osthole and P. ferulacea extract were examined on contraction induced by KCl, acetylcholine (ACh) and electrical field stimulation (EFS) and compared with propantheline and nifedipine. The acetone extract of P. ferulacea concentration-dependently relaxed ileum contraction induced by KCl (IC(50)=1.3 ± 0.25 μg/ml), ACh (IC(50)=7.7 ± 1.1 μg/ml) and EFS (IC(50)=8.8 ± 1.4 μg/ml), while, the extract at lower concentration (4 μg/ml) potentiated the ACh and EFS responses. Unlike the extract, osthole did not potentiate the ileum contraction but concentration-dependently inhibited ileum contractile responses to KCl (IC(50)=2.2 ± 0.7 μg/ml), ACh (IC(50)=2.5 ± 0.7 μg/ml) and EFS (IC(50)=2.8 ± 0.24 μg/ml). Propantheline concentration dependently inhibited the ileum response to ACh, with IC(50) value of 0.61 ± 0.09nM without affecting the KCl response. As expected, the EFS response was only partially reduced. Nifedipine (0.2-50 nM) inhibited tonic contraction induced by KCl with IC(50) value of 2.5 ± 0.8 nM but only partially inhibited the response to ACh. However, the response to EFS was reduced only by 33%. These results confirmed both potentiatory and inhibitory action of P. ferulacea extract on rat ileum contractile activity. Osthole is responsible for the inhibitory effect but potentiating components are not yet known. |
format | Online Article Text |
id | pubmed-3764677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-37646772013-09-09 Antispasmodic effects of Prangos ferulacea acetone extract and its main component osthole on ileum contraction Sadraei, H Shokoohinia, Y Sajjadi, S E Mozafari, M Res Pharm Sci Original Article Prangos ferulacea is a plant found in the Mediterranean and Middle-east regions used as carminative, anti-flatulent, emollient and antibacterial herb. It is believed that the coumarins are responsible for some of known effects of Prangos. In this research the relaxant effects of P. ferulacea coumrin rich extract as well as osthole as its main prenylated coumarins were investigated on rat ileum contraction in vitro. Relaxant effect of osthole and P. ferulacea extract were examined on contraction induced by KCl, acetylcholine (ACh) and electrical field stimulation (EFS) and compared with propantheline and nifedipine. The acetone extract of P. ferulacea concentration-dependently relaxed ileum contraction induced by KCl (IC(50)=1.3 ± 0.25 μg/ml), ACh (IC(50)=7.7 ± 1.1 μg/ml) and EFS (IC(50)=8.8 ± 1.4 μg/ml), while, the extract at lower concentration (4 μg/ml) potentiated the ACh and EFS responses. Unlike the extract, osthole did not potentiate the ileum contraction but concentration-dependently inhibited ileum contractile responses to KCl (IC(50)=2.2 ± 0.7 μg/ml), ACh (IC(50)=2.5 ± 0.7 μg/ml) and EFS (IC(50)=2.8 ± 0.24 μg/ml). Propantheline concentration dependently inhibited the ileum response to ACh, with IC(50) value of 0.61 ± 0.09nM without affecting the KCl response. As expected, the EFS response was only partially reduced. Nifedipine (0.2-50 nM) inhibited tonic contraction induced by KCl with IC(50) value of 2.5 ± 0.8 nM but only partially inhibited the response to ACh. However, the response to EFS was reduced only by 33%. These results confirmed both potentiatory and inhibitory action of P. ferulacea extract on rat ileum contractile activity. Osthole is responsible for the inhibitory effect but potentiating components are not yet known. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3764677/ /pubmed/24019823 Text en Copyright: © Journal of Research in Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Sadraei, H Shokoohinia, Y Sajjadi, S E Mozafari, M Antispasmodic effects of Prangos ferulacea acetone extract and its main component osthole on ileum contraction |
title | Antispasmodic effects of Prangos ferulacea acetone extract and its main component osthole on ileum contraction |
title_full | Antispasmodic effects of Prangos ferulacea acetone extract and its main component osthole on ileum contraction |
title_fullStr | Antispasmodic effects of Prangos ferulacea acetone extract and its main component osthole on ileum contraction |
title_full_unstemmed | Antispasmodic effects of Prangos ferulacea acetone extract and its main component osthole on ileum contraction |
title_short | Antispasmodic effects of Prangos ferulacea acetone extract and its main component osthole on ileum contraction |
title_sort | antispasmodic effects of prangos ferulacea acetone extract and its main component osthole on ileum contraction |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764677/ https://www.ncbi.nlm.nih.gov/pubmed/24019823 |
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