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Assembly of Bio-Nanoparticles for Double Controlled Drug Release
A critical limiting factor of chemotherapy is the unacceptably high toxicity. The use of nanoparticle based drug carriers has significantly reduced the side effects and facilitated the delivery of drugs. Source of the remaining side effect includes (1) the broad final in vivo distribution of the adm...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765395/ https://www.ncbi.nlm.nih.gov/pubmed/24040316 http://dx.doi.org/10.1371/journal.pone.0074679 |
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author | Huang, Wei Zhang, Jianfei Dorn, Harry C. Zhang, Chenming |
author_facet | Huang, Wei Zhang, Jianfei Dorn, Harry C. Zhang, Chenming |
author_sort | Huang, Wei |
collection | PubMed |
description | A critical limiting factor of chemotherapy is the unacceptably high toxicity. The use of nanoparticle based drug carriers has significantly reduced the side effects and facilitated the delivery of drugs. Source of the remaining side effect includes (1) the broad final in vivo distribution of the administrated nanoparticles, and (2) strong basal drug release from nanoparticles before they could reach the tumor. Despite the advances in pH-triggered release, undesirable basal drug release has been a constant challenge under in vivo conditions. In this study, functionalized single walled carbon nanohorn supported immunoliposomes were assembled for paclitaxel delivery. The immunoliposomes were formulated with polyethylene glycol, thermal stable and pH sensitive phospholipids. Each nanohorn was found to be encapsulated within one immunoliposome. Results showed a highly pH dependent release of paclitaxel in the presence of serum at body temperature with minimal basal release under physiological conditions. Upon acidification, paclitaxel was released at a steady rate over 30 days with a cumulative release of 90% of the loaded drug. The drug release results proved our hypothesized double controlled release mechanism from the nanoparticles. Other results showed the nanoparticles have doubled loading capacity compared to that of traditional liposomes and higher affinity to breast cancer cells overexpressing Her2 receptors. Internalized nanoparticles were found in lysosomes. |
format | Online Article Text |
id | pubmed-3765395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37653952013-09-13 Assembly of Bio-Nanoparticles for Double Controlled Drug Release Huang, Wei Zhang, Jianfei Dorn, Harry C. Zhang, Chenming PLoS One Research Article A critical limiting factor of chemotherapy is the unacceptably high toxicity. The use of nanoparticle based drug carriers has significantly reduced the side effects and facilitated the delivery of drugs. Source of the remaining side effect includes (1) the broad final in vivo distribution of the administrated nanoparticles, and (2) strong basal drug release from nanoparticles before they could reach the tumor. Despite the advances in pH-triggered release, undesirable basal drug release has been a constant challenge under in vivo conditions. In this study, functionalized single walled carbon nanohorn supported immunoliposomes were assembled for paclitaxel delivery. The immunoliposomes were formulated with polyethylene glycol, thermal stable and pH sensitive phospholipids. Each nanohorn was found to be encapsulated within one immunoliposome. Results showed a highly pH dependent release of paclitaxel in the presence of serum at body temperature with minimal basal release under physiological conditions. Upon acidification, paclitaxel was released at a steady rate over 30 days with a cumulative release of 90% of the loaded drug. The drug release results proved our hypothesized double controlled release mechanism from the nanoparticles. Other results showed the nanoparticles have doubled loading capacity compared to that of traditional liposomes and higher affinity to breast cancer cells overexpressing Her2 receptors. Internalized nanoparticles were found in lysosomes. Public Library of Science 2013-09-06 /pmc/articles/PMC3765395/ /pubmed/24040316 http://dx.doi.org/10.1371/journal.pone.0074679 Text en © 2013 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Huang, Wei Zhang, Jianfei Dorn, Harry C. Zhang, Chenming Assembly of Bio-Nanoparticles for Double Controlled Drug Release |
title | Assembly of Bio-Nanoparticles for Double Controlled Drug Release |
title_full | Assembly of Bio-Nanoparticles for Double Controlled Drug Release |
title_fullStr | Assembly of Bio-Nanoparticles for Double Controlled Drug Release |
title_full_unstemmed | Assembly of Bio-Nanoparticles for Double Controlled Drug Release |
title_short | Assembly of Bio-Nanoparticles for Double Controlled Drug Release |
title_sort | assembly of bio-nanoparticles for double controlled drug release |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765395/ https://www.ncbi.nlm.nih.gov/pubmed/24040316 http://dx.doi.org/10.1371/journal.pone.0074679 |
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