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Combination therapy using Pentostam and Praziquantel improves lesion healing and parasite resolution in BALB/c mice co-infected with Leishmania major and Schistosoma mansoni

BACKGROUND: Most natural host populations are exposed to a diversity of parasite communities and co-infection of hosts by multiple parasites is commonplace across a diverse range of systems. Co-infection with Leishmania major and Schistosoma mansoni may have important consequences for disease develo...

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Autores principales: Khayeka–Wandabwa, Christopher, Kutima, Helen Lydiah, Nyambati, Venny CS, Ingonga, Johnstone, Oyoo–Okoth, Elijah, Karani, Lucy Wanja, Jumba, Bernard, Githuku, Kiige Samuel, Anjili, Christopher O
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765423/
https://www.ncbi.nlm.nih.gov/pubmed/23968249
http://dx.doi.org/10.1186/1756-3305-6-244
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author Khayeka–Wandabwa, Christopher
Kutima, Helen Lydiah
Nyambati, Venny CS
Ingonga, Johnstone
Oyoo–Okoth, Elijah
Karani, Lucy Wanja
Jumba, Bernard
Githuku, Kiige Samuel
Anjili, Christopher O
author_facet Khayeka–Wandabwa, Christopher
Kutima, Helen Lydiah
Nyambati, Venny CS
Ingonga, Johnstone
Oyoo–Okoth, Elijah
Karani, Lucy Wanja
Jumba, Bernard
Githuku, Kiige Samuel
Anjili, Christopher O
author_sort Khayeka–Wandabwa, Christopher
collection PubMed
description BACKGROUND: Most natural host populations are exposed to a diversity of parasite communities and co-infection of hosts by multiple parasites is commonplace across a diverse range of systems. Co-infection with Leishmania major and Schistosoma mansoni may have important consequences for disease development, severity and transmission dynamics. Pentavalent antimonials and Praziquantel (PZQ) have been relied upon as a first line of treatment for Leishmania and Schistosoma infections respectively. However, it is not clear how combined therapy with the standard drugs will affect the host and parasite burden in concomitance. The aim of the current study was to determine the efficacy of combined chemotherapy using Pentostam and PZQ in BALB/c mice co-infected with L. major and S. mansoni. METHODS: The study used BALB/c mice infected with L. major and S. mansoni. A 3 × 4 factorial design with three parasite infection groups (Lm, Sm, Lm + Sm designated as groups infected with L. major, S. mansoni and L. major + S. mansoni, respectively) and four treatment regimens [P, PZQ, P + PZQ and PBS designating Pentostam®(GlaxoSmithKline UK), Praziquantel (Biltricide®, Bayer Ag. Leverkusen, Germany), Pentostam + Praziquantel and Phosphate buffered saline] as factors was applied. In each treatment group, there were 10 mice. Lesion development was monitored for 10 weeks. The parasite load, body weight, weight of the spleen and liver were determined between week 8 and week 10. RESULTS: Chemotherapy using the first line of treatment for L. major and S. mansoni reduced the lesion size and parasite loads but did not affect the growth response, spleen and liver. In the co-infected BALB/c mice, the use of Pentostam or PZQ did not result in any appreciable disease management. However, treatment with P + PZQ resulted in significantly (p < 0.05) larger reduction of lesions, net increase in the body weight, no changes in the spleen and liver weight and reduced Leishman-Donovan Units (LDU) and worm counts than BALB/c mice treated with Pentostam or PZQ alone. CONCLUSIONS: The present study demonstrated that the combined first line of treatment is a more effective strategy in managing co-infection of L. major and S. mansoni in BALB/c mice.
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spelling pubmed-37654232013-09-10 Combination therapy using Pentostam and Praziquantel improves lesion healing and parasite resolution in BALB/c mice co-infected with Leishmania major and Schistosoma mansoni Khayeka–Wandabwa, Christopher Kutima, Helen Lydiah Nyambati, Venny CS Ingonga, Johnstone Oyoo–Okoth, Elijah Karani, Lucy Wanja Jumba, Bernard Githuku, Kiige Samuel Anjili, Christopher O Parasit Vectors Research BACKGROUND: Most natural host populations are exposed to a diversity of parasite communities and co-infection of hosts by multiple parasites is commonplace across a diverse range of systems. Co-infection with Leishmania major and Schistosoma mansoni may have important consequences for disease development, severity and transmission dynamics. Pentavalent antimonials and Praziquantel (PZQ) have been relied upon as a first line of treatment for Leishmania and Schistosoma infections respectively. However, it is not clear how combined therapy with the standard drugs will affect the host and parasite burden in concomitance. The aim of the current study was to determine the efficacy of combined chemotherapy using Pentostam and PZQ in BALB/c mice co-infected with L. major and S. mansoni. METHODS: The study used BALB/c mice infected with L. major and S. mansoni. A 3 × 4 factorial design with three parasite infection groups (Lm, Sm, Lm + Sm designated as groups infected with L. major, S. mansoni and L. major + S. mansoni, respectively) and four treatment regimens [P, PZQ, P + PZQ and PBS designating Pentostam®(GlaxoSmithKline UK), Praziquantel (Biltricide®, Bayer Ag. Leverkusen, Germany), Pentostam + Praziquantel and Phosphate buffered saline] as factors was applied. In each treatment group, there were 10 mice. Lesion development was monitored for 10 weeks. The parasite load, body weight, weight of the spleen and liver were determined between week 8 and week 10. RESULTS: Chemotherapy using the first line of treatment for L. major and S. mansoni reduced the lesion size and parasite loads but did not affect the growth response, spleen and liver. In the co-infected BALB/c mice, the use of Pentostam or PZQ did not result in any appreciable disease management. However, treatment with P + PZQ resulted in significantly (p < 0.05) larger reduction of lesions, net increase in the body weight, no changes in the spleen and liver weight and reduced Leishman-Donovan Units (LDU) and worm counts than BALB/c mice treated with Pentostam or PZQ alone. CONCLUSIONS: The present study demonstrated that the combined first line of treatment is a more effective strategy in managing co-infection of L. major and S. mansoni in BALB/c mice. BioMed Central 2013-08-22 /pmc/articles/PMC3765423/ /pubmed/23968249 http://dx.doi.org/10.1186/1756-3305-6-244 Text en Copyright © 2013 Khayeka–Wandabwa et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Khayeka–Wandabwa, Christopher
Kutima, Helen Lydiah
Nyambati, Venny CS
Ingonga, Johnstone
Oyoo–Okoth, Elijah
Karani, Lucy Wanja
Jumba, Bernard
Githuku, Kiige Samuel
Anjili, Christopher O
Combination therapy using Pentostam and Praziquantel improves lesion healing and parasite resolution in BALB/c mice co-infected with Leishmania major and Schistosoma mansoni
title Combination therapy using Pentostam and Praziquantel improves lesion healing and parasite resolution in BALB/c mice co-infected with Leishmania major and Schistosoma mansoni
title_full Combination therapy using Pentostam and Praziquantel improves lesion healing and parasite resolution in BALB/c mice co-infected with Leishmania major and Schistosoma mansoni
title_fullStr Combination therapy using Pentostam and Praziquantel improves lesion healing and parasite resolution in BALB/c mice co-infected with Leishmania major and Schistosoma mansoni
title_full_unstemmed Combination therapy using Pentostam and Praziquantel improves lesion healing and parasite resolution in BALB/c mice co-infected with Leishmania major and Schistosoma mansoni
title_short Combination therapy using Pentostam and Praziquantel improves lesion healing and parasite resolution in BALB/c mice co-infected with Leishmania major and Schistosoma mansoni
title_sort combination therapy using pentostam and praziquantel improves lesion healing and parasite resolution in balb/c mice co-infected with leishmania major and schistosoma mansoni
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765423/
https://www.ncbi.nlm.nih.gov/pubmed/23968249
http://dx.doi.org/10.1186/1756-3305-6-244
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