Characterization of a novel polyclonal anti-hypusine antibody

The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we pr...

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Detalles Bibliográficos
Autores principales: Nishiki, Yurika, Farb, Thomas B, Friedrich, Jessica, Bokvist, Krister, Mirmira, Raghavendra G, Maier, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2013
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765601/
https://www.ncbi.nlm.nih.gov/pubmed/24024105
http://dx.doi.org/10.1186/2193-1801-2-421
Descripción
Sumario:The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A.

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