Characterization of a novel polyclonal anti-hypusine antibody

The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we pr...

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Autores principales: Nishiki, Yurika, Farb, Thomas B, Friedrich, Jessica, Bokvist, Krister, Mirmira, Raghavendra G, Maier, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2013
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765601/
https://www.ncbi.nlm.nih.gov/pubmed/24024105
http://dx.doi.org/10.1186/2193-1801-2-421
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author Nishiki, Yurika
Farb, Thomas B
Friedrich, Jessica
Bokvist, Krister
Mirmira, Raghavendra G
Maier, Bernhard
author_facet Nishiki, Yurika
Farb, Thomas B
Friedrich, Jessica
Bokvist, Krister
Mirmira, Raghavendra G
Maier, Bernhard
author_sort Nishiki, Yurika
collection PubMed
description The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A.
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spelling pubmed-37656012013-09-10 Characterization of a novel polyclonal anti-hypusine antibody Nishiki, Yurika Farb, Thomas B Friedrich, Jessica Bokvist, Krister Mirmira, Raghavendra G Maier, Bernhard Springerplus Technical Note The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A. Springer International Publishing 2013-08-29 /pmc/articles/PMC3765601/ /pubmed/24024105 http://dx.doi.org/10.1186/2193-1801-2-421 Text en © Nishiki et al.; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Note
Nishiki, Yurika
Farb, Thomas B
Friedrich, Jessica
Bokvist, Krister
Mirmira, Raghavendra G
Maier, Bernhard
Characterization of a novel polyclonal anti-hypusine antibody
title Characterization of a novel polyclonal anti-hypusine antibody
title_full Characterization of a novel polyclonal anti-hypusine antibody
title_fullStr Characterization of a novel polyclonal anti-hypusine antibody
title_full_unstemmed Characterization of a novel polyclonal anti-hypusine antibody
title_short Characterization of a novel polyclonal anti-hypusine antibody
title_sort characterization of a novel polyclonal anti-hypusine antibody
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765601/
https://www.ncbi.nlm.nih.gov/pubmed/24024105
http://dx.doi.org/10.1186/2193-1801-2-421
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