Cargando…
A multiple-alignment based primer design algorithm for genetically highly variable DNA targets
BACKGROUND: Primer design for highly variable DNA sequences is difficult, and experimental success requires attention to many interacting constraints. The advent of next-generation sequencing methods allows the investigation of rare variants otherwise hidden deep in large populations, but requires a...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2013
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765731/ https://www.ncbi.nlm.nih.gov/pubmed/23965160 http://dx.doi.org/10.1186/1471-2105-14-255 |