Cargando…

Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells

BACKGROUND: Ssanghwa-tang (SHT) is a widely used medication for the treatment of fatigue, pain, inflammation, hypothermia, erectile dysfunction, cancer, and osteoporosis in Asia, however, role of SHT on the melanin synthesis has not been checked previously. Thus, the present study was designed to de...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Aeyung, Yim, Nam-Hui, Im, Minju, Jung, Young Pil, Liang, Chun, Cho, Won-Kyung, Ma, Jin Yeul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765811/
https://www.ncbi.nlm.nih.gov/pubmed/23981281
http://dx.doi.org/10.1186/1472-6882-13-214
_version_ 1782283396553113600
author Kim, Aeyung
Yim, Nam-Hui
Im, Minju
Jung, Young Pil
Liang, Chun
Cho, Won-Kyung
Ma, Jin Yeul
author_facet Kim, Aeyung
Yim, Nam-Hui
Im, Minju
Jung, Young Pil
Liang, Chun
Cho, Won-Kyung
Ma, Jin Yeul
author_sort Kim, Aeyung
collection PubMed
description BACKGROUND: Ssanghwa-tang (SHT) is a widely used medication for the treatment of fatigue, pain, inflammation, hypothermia, erectile dysfunction, cancer, and osteoporosis in Asia, however, role of SHT on the melanin synthesis has not been checked previously. Thus, the present study was designed to determine the effect of SHT on α-melanocyte stimulating hormone (α-MSH)-induced melanogensis and its mechanisms of action in murine B16F10 melanoma cells. METHOD: Cellular melanin content and tyrosinase activity in murine B16F10 melanoma cells were determined after α-MSH stimulation with or without pre-treatment of SHT at the concentration of 250 and 500 μg/ml. Expression level of tyrosinase, tyrosinase-related protein 1 (TRP-1), TRP-2, microphthalmia-associated transcription factor (MITF), and activation of c-AMP-dependent protein kinase (PKA), c-AMP-related element binding protein (CREB), and mitogen-activated protein kinases (MAPKs) were examined by Western blot analysis. RESULTS: SHT significantly inhibited α-MSH-induced melanin synthesis and tyrosinase activity, and also decreased α-MSH-induced expression of MITF, tyrosinase, and TRP-1. In addition, SHT remarkably suppressed tyrosinase, CRE, and MITF luciferase reporter activity in a resting state as well as in α-MSH-stimulating condition. Phosphorylation of p38 MAPK by α-MSH stimulation was efficiently blocked by SHT pre-treatment. Moreover, SHT as an herbal cocktail showed synergistic anti-melanogenic effect compared with that of each single constituent herb. CONCLUSION: SHT efficiently inhibited c-AMP-induced melanin synthesis in B16F10 cells via suppression of PKA and p38 MAPK signaling pathways and subsequently decreased the level of CREB phosphorylation, MITF, and melanogenic enzymes. These results indicate that SHT may be useful as herbal medicine for treating hyperpigmentation and cosmetics as a skin-whitening agent.
format Online
Article
Text
id pubmed-3765811
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-37658112013-09-08 Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells Kim, Aeyung Yim, Nam-Hui Im, Minju Jung, Young Pil Liang, Chun Cho, Won-Kyung Ma, Jin Yeul BMC Complement Altern Med Research Article BACKGROUND: Ssanghwa-tang (SHT) is a widely used medication for the treatment of fatigue, pain, inflammation, hypothermia, erectile dysfunction, cancer, and osteoporosis in Asia, however, role of SHT on the melanin synthesis has not been checked previously. Thus, the present study was designed to determine the effect of SHT on α-melanocyte stimulating hormone (α-MSH)-induced melanogensis and its mechanisms of action in murine B16F10 melanoma cells. METHOD: Cellular melanin content and tyrosinase activity in murine B16F10 melanoma cells were determined after α-MSH stimulation with or without pre-treatment of SHT at the concentration of 250 and 500 μg/ml. Expression level of tyrosinase, tyrosinase-related protein 1 (TRP-1), TRP-2, microphthalmia-associated transcription factor (MITF), and activation of c-AMP-dependent protein kinase (PKA), c-AMP-related element binding protein (CREB), and mitogen-activated protein kinases (MAPKs) were examined by Western blot analysis. RESULTS: SHT significantly inhibited α-MSH-induced melanin synthesis and tyrosinase activity, and also decreased α-MSH-induced expression of MITF, tyrosinase, and TRP-1. In addition, SHT remarkably suppressed tyrosinase, CRE, and MITF luciferase reporter activity in a resting state as well as in α-MSH-stimulating condition. Phosphorylation of p38 MAPK by α-MSH stimulation was efficiently blocked by SHT pre-treatment. Moreover, SHT as an herbal cocktail showed synergistic anti-melanogenic effect compared with that of each single constituent herb. CONCLUSION: SHT efficiently inhibited c-AMP-induced melanin synthesis in B16F10 cells via suppression of PKA and p38 MAPK signaling pathways and subsequently decreased the level of CREB phosphorylation, MITF, and melanogenic enzymes. These results indicate that SHT may be useful as herbal medicine for treating hyperpigmentation and cosmetics as a skin-whitening agent. BioMed Central 2013-08-28 /pmc/articles/PMC3765811/ /pubmed/23981281 http://dx.doi.org/10.1186/1472-6882-13-214 Text en Copyright © 2013 Kim et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Aeyung
Yim, Nam-Hui
Im, Minju
Jung, Young Pil
Liang, Chun
Cho, Won-Kyung
Ma, Jin Yeul
Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells
title Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells
title_full Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells
title_fullStr Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells
title_full_unstemmed Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells
title_short Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells
title_sort ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 mapk and pka signaling pathways in b16f10 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765811/
https://www.ncbi.nlm.nih.gov/pubmed/23981281
http://dx.doi.org/10.1186/1472-6882-13-214
work_keys_str_mv AT kimaeyung ssanghwatanganorientalherbalcocktailexertsantimelanogenicactivitybysuppressionofthep38mapkandpkasignalingpathwaysinb16f10cells
AT yimnamhui ssanghwatanganorientalherbalcocktailexertsantimelanogenicactivitybysuppressionofthep38mapkandpkasignalingpathwaysinb16f10cells
AT imminju ssanghwatanganorientalherbalcocktailexertsantimelanogenicactivitybysuppressionofthep38mapkandpkasignalingpathwaysinb16f10cells
AT jungyoungpil ssanghwatanganorientalherbalcocktailexertsantimelanogenicactivitybysuppressionofthep38mapkandpkasignalingpathwaysinb16f10cells
AT liangchun ssanghwatanganorientalherbalcocktailexertsantimelanogenicactivitybysuppressionofthep38mapkandpkasignalingpathwaysinb16f10cells
AT chowonkyung ssanghwatanganorientalherbalcocktailexertsantimelanogenicactivitybysuppressionofthep38mapkandpkasignalingpathwaysinb16f10cells
AT majinyeul ssanghwatanganorientalherbalcocktailexertsantimelanogenicactivitybysuppressionofthep38mapkandpkasignalingpathwaysinb16f10cells