Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours – IPO8 and RPL4 are reliable reference genes

BACKGROUND: To ensure a correct interpretation of results obtained with quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR), it is critical to normalize to a reference gene with stable mRNA expression in the tissue of interest. GADPH is widely used as a reference gene in...

Descripción completa

Detalles Bibliográficos
Autores principales: Kolkova, Zuzana, Arakelyan, Arsen, Casslén, Bertil, Hansson, Stefan, Kriegova, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766134/
https://www.ncbi.nlm.nih.gov/pubmed/24001041
http://dx.doi.org/10.1186/1757-2215-6-60

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766134/
https://www.ncbi.nlm.nih.gov/pubmed/24001041
http://dx.doi.org/10.1186/1757-2215-6-60

Ejemplares similares