Cargando…

Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours – IPO8 and RPL4 are reliable reference genes

BACKGROUND: To ensure a correct interpretation of results obtained with quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR), it is critical to normalize to a reference gene with stable mRNA expression in the tissue of interest. GADPH is widely used as a reference gene in...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kolkova, Zuzana, Arakelyan, Arsen, Casslén, Bertil, Hansson, Stefan, Kriegova, Eva
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766134/ https://www.ncbi.nlm.nih.gov/pubmed/24001041 http://dx.doi.org/10.1186/1757-2215-6-60

Ejemplares similares

PSMB2 and RPL32 are suitable denominators to normalize gene expression profiles in bronchoalveolar cells
por: Kriegova, Eva, et al.
Publicado: (2008)

The G protein-coupled estrogen receptor 1 (GPER/GPR30) does not predict survival in patients with ovarian cancer
por: Kolkova, Zuzana, et al.
Publicado: (2012)

Is Rescuer Cardiopulmonary Resuscitation Jeopardised by Previous Fatiguing Exercise?
por: Abraldes, J. Arturo, et al.
Publicado: (2020)

Government support for alcohol industry: promoting exports, jeopardising global health?
por: Collin, Jeff, et al.
Publicado: (2014)

Costly mistakes: Why and when spelling errors in resumes jeopardise interview chances
por: Sterkens, Philippe, et al.
Publicado: (2023)

Evidence of Horizontal Gene Transfer of 50S Ribosomal Genes rplB, rplD, and rplY in Neisseria gonorrhoeae
por: Manoharan-Basil, Sheeba Santhini, et al.
Publicado: (2021)

Identification of Importin 8 (IPO8) as the most accurate reference gene for the clinicopathological analysis of lung specimens
por: Nguewa, Paul A, et al.
Publicado: (2008)

High-profit IPO strategies: finding breakout IPOs for investors and traders
por: Taulli, Tom, et al.
Publicado: (2013)

Long-term environmental metal exposure is associated with hypomethylation of CpG sites in NFKB1 and other genes related to oncogenesis
por: Stepanyan, Ani, et al.
Publicado: (2023)

Correction: Long-term environmental metal exposure is associated with hypomethylation of CpG sites in NFKB1 and other genes related to oncogenesis
por: Stepanyan, Ani, et al.
Publicado: (2023)

Corporate governance in IPO firms
por: Lehmann, Erik E, et al.
Publicado: (2020)

Could anti-CD20 therapy jeopardise the efficacy of a SARS-CoV-2 vaccine?
por: Houot, Roch, et al.
Publicado: (2020)

Epstein-Barr Virus Limits the Accumulation of IPO7, an Essential Gene Product
por: Yang, Ya-Chun, et al.
Publicado: (2021)

Genetic Variants of RPL5 and RPL9 Genes among Saudi Patients Diagnosed with Thrombosis
por: Hassan, Fathelrahman Mahdi, et al.
Publicado: (2021)

Latent class analysis of IPOs in the Nordics
por: Bask, Mikael, et al.
Publicado: (2021)

Translation, cultural adaptation, and validation of the Integrated Palliative Outcome Scale-renal (IPOS-r) to Czech
por: Křemenová, Zuzana, et al.
Publicado: (2022)

Validity and reliability of the English and translated Chinese versions of the Integrated Palliative care Outcome Scale (IPOS) in Singapore
por: Long, Victoria Jane En, et al.
Publicado: (2021)

Correction: Latent class analysis of IPOs in the Nordics
Publicado: (2023)

Special Issue “Disentangling Mechanisms of Genomic Regulation of Cell Functions at the Gene Level”
por: Binder, Hans, et al.
Publicado: (2020)

Arabidopsis paralogous genes RPL23aA and RPL23aB encode functionally equivalent proteins
por: Xiong, Wei, et al.
Publicado: (2020)

Discovery of the rpl10 Gene in Diverse Plant Mitochondrial Genomes and Its Probable Replacement by the Nuclear Gene for Chloroplast RPL10 in Two Lineages of Angiosperms
por: Kubo, Nakao, et al.
Publicado: (2010)

RPL-4 and RPL-9 –Mediated Ribosome Purifications Facilitate the Efficient Analysis of Gene Expression in Caenorhabditis elegans Germ Cells
por: Nousch, Marco
Publicado: (2020)

Regulation A+ and Other Alternatives to a Traditional IPO
por: Feldman, David
Publicado: (2018)

IPO: a tool for automated optimization of XCMS parameters
por: Libiseller, Gunnar, et al.
Publicado: (2015)

Unicorns’ growth and financial flexibility before and after the IPO
por: Zhou, Man, et al.
Publicado: (2023)

Can transthoracic echocardiography be used to a greater extent in the diagnostics of infective endocarditis to avoid unnecessary transoesophageal examinations without jeopardising accuracy?
por: Damlin, Anna, et al.
Publicado: (2023)

Identification of Reliable Reference Genes for Quantification of MicroRNAs in Serum Samples of Sulfur Mustard-Exposed Veterans
por: Gharbi, Sedigheh, et al.
Publicado: (2015)

Reliable reference genes for the quantification of mRNA in human T-cells and PBMCs stimulated with live influenza virus
por: Roy, Justin G., et al.
Publicado: (2020)

Involvement of polymorphisms of the nerve growth factor and its receptor encoding genes in the etiopathogenesis of ischemic stroke
por: Stepanyan, Ani, et al.
Publicado: (2018)

The RPL/RPS Gene Signature of Melanoma CTCs Associates with Brain Metastasis
por: Bowley, Tetiana Y., et al.
Publicado: (2022)

COVID-19: Fear of pandemic and short-term IPO performance
por: Mazumder, Sharif, et al.
Publicado: (2021)

Nuclear Transporter IPO13 Is Central to Efficient Neuronal Differentiation
por: Gajewska, Katarzyna A., et al.
Publicado: (2022)

Competitive threat and strategic disclosure during the IPO quiet period
por: Qiu, Yetaotao, et al.
Publicado: (2022)

Reference-based QUantification Of gene Dispensability (QUOD)
por: Sielemann, Katharina, et al.
Publicado: (2021)

Analysis of the stability of the reference genes GAPDH, SDHA and RPL-19 in sheep from a semi-arid region infected by gastrointestinal nematodes
por: Lucas Nascimento Souza, Jorge, et al.
Publicado: (2023)

Telomere Maintenance Pathway Activity Analysis Enables Tissue- and Gene-Level Inferences
por: Nersisyan, Lilit, et al.
Publicado: (2021)

WGS-based telomere length analysis in Dutch family trios implicates stronger maternal inheritance and a role for RRM1 gene
por: Nersisyan, Lilit, et al.
Publicado: (2019)

Selection of Reliable Reference Genes for Analysis of Gene Expression in Spinal Cord during Rat Postnatal Development and after Injury
por: Košuth, Ján, et al.
Publicado: (2019)

Dosage Sensitivity of RPL9 and Concerted Evolution of Ribosomal Protein Genes in Plants
por: Devis, Deborah, et al.
Publicado: (2015)

High preoperative blood levels of HE4 predicts poor prognosis in patients with ovarian cancer
por: Kalapotharakos, Grigorios, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_b7n038a0hk2rrq0kc9krhnffh4