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Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours – IPO8 and RPL4 are reliable reference genes

BACKGROUND: To ensure a correct interpretation of results obtained with quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR), it is critical to normalize to a reference gene with stable mRNA expression in the tissue of interest. GADPH is widely used as a reference gene in...

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Detalles Bibliográficos
Autores principales:	Kolkova, Zuzana, Arakelyan, Arsen, Casslén, Bertil, Hansson, Stefan, Kriegova, Eva
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766134/ https://www.ncbi.nlm.nih.gov/pubmed/24001041 http://dx.doi.org/10.1186/1757-2215-6-60

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