Cargando…
Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012
BACKGROUND: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766201/ https://www.ncbi.nlm.nih.gov/pubmed/24138669 http://dx.doi.org/10.1186/1471-2334-13-409 |
_version_ | 1782283486450679808 |
---|---|
author | Lai, Jana YR Cook, Heather Yip, Teem-Wing Berthelsen, Jeanette Gourley, Stephen Krause, Vicki Smith, Helen Leach, Amanda J Smith-Vaughan, Heidi |
author_facet | Lai, Jana YR Cook, Heather Yip, Teem-Wing Berthelsen, Jeanette Gourley, Stephen Krause, Vicki Smith, Helen Leach, Amanda J Smith-Vaughan, Heidi |
author_sort | Lai, Jana YR |
collection | PubMed |
description | BACKGROUND: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. METHODS: 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). RESULTS: Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. CONCLUSIONS: During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age. |
format | Online Article Text |
id | pubmed-3766201 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-37662012013-09-08 Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012 Lai, Jana YR Cook, Heather Yip, Teem-Wing Berthelsen, Jeanette Gourley, Stephen Krause, Vicki Smith, Helen Leach, Amanda J Smith-Vaughan, Heidi BMC Infect Dis Research Article BACKGROUND: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. METHODS: 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). RESULTS: Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. CONCLUSIONS: During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age. BioMed Central 2013-09-03 /pmc/articles/PMC3766201/ /pubmed/24138669 http://dx.doi.org/10.1186/1471-2334-13-409 Text en Copyright © 2013 Lai et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lai, Jana YR Cook, Heather Yip, Teem-Wing Berthelsen, Jeanette Gourley, Stephen Krause, Vicki Smith, Helen Leach, Amanda J Smith-Vaughan, Heidi Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012 |
title | Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012 |
title_full | Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012 |
title_fullStr | Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012 |
title_full_unstemmed | Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012 |
title_short | Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012 |
title_sort | surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central australia 2010–2012 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766201/ https://www.ncbi.nlm.nih.gov/pubmed/24138669 http://dx.doi.org/10.1186/1471-2334-13-409 |
work_keys_str_mv | AT laijanayr surveillanceofpneumococcalserotype1carriageduringanoutbreakofserotype1invasivepneumococcaldiseaseincentralaustralia20102012 AT cookheather surveillanceofpneumococcalserotype1carriageduringanoutbreakofserotype1invasivepneumococcaldiseaseincentralaustralia20102012 AT yipteemwing surveillanceofpneumococcalserotype1carriageduringanoutbreakofserotype1invasivepneumococcaldiseaseincentralaustralia20102012 AT berthelsenjeanette surveillanceofpneumococcalserotype1carriageduringanoutbreakofserotype1invasivepneumococcaldiseaseincentralaustralia20102012 AT gourleystephen surveillanceofpneumococcalserotype1carriageduringanoutbreakofserotype1invasivepneumococcaldiseaseincentralaustralia20102012 AT krausevicki surveillanceofpneumococcalserotype1carriageduringanoutbreakofserotype1invasivepneumococcaldiseaseincentralaustralia20102012 AT smithhelen surveillanceofpneumococcalserotype1carriageduringanoutbreakofserotype1invasivepneumococcaldiseaseincentralaustralia20102012 AT leachamandaj surveillanceofpneumococcalserotype1carriageduringanoutbreakofserotype1invasivepneumococcaldiseaseincentralaustralia20102012 AT smithvaughanheidi surveillanceofpneumococcalserotype1carriageduringanoutbreakofserotype1invasivepneumococcaldiseaseincentralaustralia20102012 |