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Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012

BACKGROUND: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the...

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Autores principales: Lai, Jana YR, Cook, Heather, Yip, Teem-Wing, Berthelsen, Jeanette, Gourley, Stephen, Krause, Vicki, Smith, Helen, Leach, Amanda J, Smith-Vaughan, Heidi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766201/
https://www.ncbi.nlm.nih.gov/pubmed/24138669
http://dx.doi.org/10.1186/1471-2334-13-409
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author Lai, Jana YR
Cook, Heather
Yip, Teem-Wing
Berthelsen, Jeanette
Gourley, Stephen
Krause, Vicki
Smith, Helen
Leach, Amanda J
Smith-Vaughan, Heidi
author_facet Lai, Jana YR
Cook, Heather
Yip, Teem-Wing
Berthelsen, Jeanette
Gourley, Stephen
Krause, Vicki
Smith, Helen
Leach, Amanda J
Smith-Vaughan, Heidi
author_sort Lai, Jana YR
collection PubMed
description BACKGROUND: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. METHODS: 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). RESULTS: Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. CONCLUSIONS: During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age.
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spelling pubmed-37662012013-09-08 Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012 Lai, Jana YR Cook, Heather Yip, Teem-Wing Berthelsen, Jeanette Gourley, Stephen Krause, Vicki Smith, Helen Leach, Amanda J Smith-Vaughan, Heidi BMC Infect Dis Research Article BACKGROUND: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. METHODS: 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). RESULTS: Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. CONCLUSIONS: During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age. BioMed Central 2013-09-03 /pmc/articles/PMC3766201/ /pubmed/24138669 http://dx.doi.org/10.1186/1471-2334-13-409 Text en Copyright © 2013 Lai et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lai, Jana YR
Cook, Heather
Yip, Teem-Wing
Berthelsen, Jeanette
Gourley, Stephen
Krause, Vicki
Smith, Helen
Leach, Amanda J
Smith-Vaughan, Heidi
Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012
title Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012
title_full Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012
title_fullStr Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012
title_full_unstemmed Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012
title_short Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012
title_sort surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central australia 2010–2012
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766201/
https://www.ncbi.nlm.nih.gov/pubmed/24138669
http://dx.doi.org/10.1186/1471-2334-13-409
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