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Influence of Bovine Serum Albumin on the Antibacterial Activity of Endodontic Irrigants against Enterococcus Faecalis
INTRODUCTION: It has been demonstrated that organic content of the root canals can influence the antimicrobial capability of chemical irrigants. The aim of this study was to evaluate the effect of bovine serum albumin (BSA), as an organic material, on the antimicrobial activity of several intracanal...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Iranian Center for Endodontic Research
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766621/ https://www.ncbi.nlm.nih.gov/pubmed/24019835 |
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author | Khedmat, Sedigheh Aligholi, Marziyeh Sadeghi, Samaneh |
author_facet | Khedmat, Sedigheh Aligholi, Marziyeh Sadeghi, Samaneh |
author_sort | Khedmat, Sedigheh |
collection | PubMed |
description | INTRODUCTION: It has been demonstrated that organic content of the root canals can influence the antimicrobial capability of chemical irrigants. The aim of this study was to evaluate the effect of bovine serum albumin (BSA), as an organic material, on the antimicrobial activity of several intracanal irrigants. MATERIALS AND METHODS: Bactericidal activity of Ethylenediaminetetraacetic acid (EDTA) 17%, citric acid 10%, Sodium hypochlorite (NaOCl) 5.25%, Chlorhexidine 0.2% (CHX), Smear Clear and Cetrimide 0.5% were tested by means of dilution-neutralization method. Contact times were 10 and 30 seconds, 5, 10, 30, 60 minutes and 24 hours. First 950 λ of the medicament was mixed with 50 λ of the bacterial suspension in an Eppendorf test tube. The suspensions were thoroughly mixed. Sterile water served as negative controls. After each contact time, 100 λ of samples was transferred to the Eppendorf test tubes which contained neutralizers. After 5 minutes, 50 λ of serial dilutions were cultured on brain heart infusion agar and incubated in aerobic conditions. Then colonies were counted and reported as cfu/mL. In half of the samples, medicaments were suspended in BSA 0.5% 30 minutes before examination to assess its possible inhibitory effect on the antibacterial activity. RESULTS: NaOCl 5.25%, Cetrimide 0.5% and Smear Clear showed bactericidal activity within seconds after the incubation. BSA had no inhibitory effect on bactericidal activity of these three medicaments. CHX took 5 and10 minutes to kill all bacterial cells in the absence and presence of BSA, respectively. Citric acid and EDTA showed the least antibacterial activity. CONCLUSION: In this study, NaOCl 5.25%, Cetrimide 0.5% and Smear Clear were significantly more effective against E. faecalis than EDTA 17% and citric acid 10% in the presence and absence of BSA. Also, in the presence of BSA, bactericidal activity of CHX 0.2% against E. faecalis was significantly more than EDTA after 10 and 30 minutes of contact time. EDTA and citric acid showed the least bactericidal activity. [Iranian Endodontic Journal 2009;4(4):139-43] |
format | Online Article Text |
id | pubmed-3766621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Iranian Center for Endodontic Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-37666212013-09-09 Influence of Bovine Serum Albumin on the Antibacterial Activity of Endodontic Irrigants against Enterococcus Faecalis Khedmat, Sedigheh Aligholi, Marziyeh Sadeghi, Samaneh Iran Endod J Original Article INTRODUCTION: It has been demonstrated that organic content of the root canals can influence the antimicrobial capability of chemical irrigants. The aim of this study was to evaluate the effect of bovine serum albumin (BSA), as an organic material, on the antimicrobial activity of several intracanal irrigants. MATERIALS AND METHODS: Bactericidal activity of Ethylenediaminetetraacetic acid (EDTA) 17%, citric acid 10%, Sodium hypochlorite (NaOCl) 5.25%, Chlorhexidine 0.2% (CHX), Smear Clear and Cetrimide 0.5% were tested by means of dilution-neutralization method. Contact times were 10 and 30 seconds, 5, 10, 30, 60 minutes and 24 hours. First 950 λ of the medicament was mixed with 50 λ of the bacterial suspension in an Eppendorf test tube. The suspensions were thoroughly mixed. Sterile water served as negative controls. After each contact time, 100 λ of samples was transferred to the Eppendorf test tubes which contained neutralizers. After 5 minutes, 50 λ of serial dilutions were cultured on brain heart infusion agar and incubated in aerobic conditions. Then colonies were counted and reported as cfu/mL. In half of the samples, medicaments were suspended in BSA 0.5% 30 minutes before examination to assess its possible inhibitory effect on the antibacterial activity. RESULTS: NaOCl 5.25%, Cetrimide 0.5% and Smear Clear showed bactericidal activity within seconds after the incubation. BSA had no inhibitory effect on bactericidal activity of these three medicaments. CHX took 5 and10 minutes to kill all bacterial cells in the absence and presence of BSA, respectively. Citric acid and EDTA showed the least antibacterial activity. CONCLUSION: In this study, NaOCl 5.25%, Cetrimide 0.5% and Smear Clear were significantly more effective against E. faecalis than EDTA 17% and citric acid 10% in the presence and absence of BSA. Also, in the presence of BSA, bactericidal activity of CHX 0.2% against E. faecalis was significantly more than EDTA after 10 and 30 minutes of contact time. EDTA and citric acid showed the least bactericidal activity. [Iranian Endodontic Journal 2009;4(4):139-43] Iranian Center for Endodontic Research 2009-10-10 2009 /pmc/articles/PMC3766621/ /pubmed/24019835 Text en © 2009, Iranian Center for Endodontic Research This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Khedmat, Sedigheh Aligholi, Marziyeh Sadeghi, Samaneh Influence of Bovine Serum Albumin on the Antibacterial Activity of Endodontic Irrigants against Enterococcus Faecalis |
title | Influence of Bovine Serum Albumin on the Antibacterial Activity of Endodontic Irrigants against Enterococcus Faecalis |
title_full | Influence of Bovine Serum Albumin on the Antibacterial Activity of Endodontic Irrigants against Enterococcus Faecalis |
title_fullStr | Influence of Bovine Serum Albumin on the Antibacterial Activity of Endodontic Irrigants against Enterococcus Faecalis |
title_full_unstemmed | Influence of Bovine Serum Albumin on the Antibacterial Activity of Endodontic Irrigants against Enterococcus Faecalis |
title_short | Influence of Bovine Serum Albumin on the Antibacterial Activity of Endodontic Irrigants against Enterococcus Faecalis |
title_sort | influence of bovine serum albumin on the antibacterial activity of endodontic irrigants against enterococcus faecalis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766621/ https://www.ncbi.nlm.nih.gov/pubmed/24019835 |
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