Cargando…

Development of Synechocystis sp. PCC 6803 as a Phototrophic Cell Factory

Cyanobacteria (blue-green algae) play profound roles in ecology and biogeochemistry. One model cyanobacterial species is the unicellular cyanobacterium Synechocystis sp. PCC 6803. This species is highly amenable to genetic modification. Its genome has been sequenced and many systems biology and mole...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Yi, You, Le, Liu, Dianyi, Hollinshead, Whitney, Tang, Yinjie J., Zhang, Fuzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766872/
https://www.ncbi.nlm.nih.gov/pubmed/23945601
http://dx.doi.org/10.3390/md11082894
_version_ 1782477336443092992
author Yu, Yi
You, Le
Liu, Dianyi
Hollinshead, Whitney
Tang, Yinjie J.
Zhang, Fuzhong
author_facet Yu, Yi
You, Le
Liu, Dianyi
Hollinshead, Whitney
Tang, Yinjie J.
Zhang, Fuzhong
author_sort Yu, Yi
collection PubMed
description Cyanobacteria (blue-green algae) play profound roles in ecology and biogeochemistry. One model cyanobacterial species is the unicellular cyanobacterium Synechocystis sp. PCC 6803. This species is highly amenable to genetic modification. Its genome has been sequenced and many systems biology and molecular biology tools are available to study this bacterium. Recently, researchers have put significant efforts into understanding and engineering this bacterium to produce chemicals and biofuels from sunlight and CO(2). To demonstrate our perspective on the application of this cyanobacterium as a photosynthesis-based chassis, we summarize the recent research on Synechocystis 6803 by focusing on five topics: rate-limiting factors for cell cultivation; molecular tools for genetic modifications; high-throughput system biology for genome wide analysis; metabolic modeling for physiological prediction and rational metabolic engineering; and applications in producing diverse chemicals. We also discuss the particular challenges for systems analysis and engineering applications of this microorganism, including precise characterization of versatile cell metabolism, improvement of product rates and titers, bioprocess scale-up, and product recovery. Although much progress has been achieved in the development of Synechocystis 6803 as a phototrophic cell factory, the biotechnology for “Compounds from Synechocystis” is still significantly lagging behind those for heterotrophic microbes (e.g., Escherichia coli).
format Online
Article
Text
id pubmed-3766872
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-37668722013-09-09 Development of Synechocystis sp. PCC 6803 as a Phototrophic Cell Factory Yu, Yi You, Le Liu, Dianyi Hollinshead, Whitney Tang, Yinjie J. Zhang, Fuzhong Mar Drugs Review Cyanobacteria (blue-green algae) play profound roles in ecology and biogeochemistry. One model cyanobacterial species is the unicellular cyanobacterium Synechocystis sp. PCC 6803. This species is highly amenable to genetic modification. Its genome has been sequenced and many systems biology and molecular biology tools are available to study this bacterium. Recently, researchers have put significant efforts into understanding and engineering this bacterium to produce chemicals and biofuels from sunlight and CO(2). To demonstrate our perspective on the application of this cyanobacterium as a photosynthesis-based chassis, we summarize the recent research on Synechocystis 6803 by focusing on five topics: rate-limiting factors for cell cultivation; molecular tools for genetic modifications; high-throughput system biology for genome wide analysis; metabolic modeling for physiological prediction and rational metabolic engineering; and applications in producing diverse chemicals. We also discuss the particular challenges for systems analysis and engineering applications of this microorganism, including precise characterization of versatile cell metabolism, improvement of product rates and titers, bioprocess scale-up, and product recovery. Although much progress has been achieved in the development of Synechocystis 6803 as a phototrophic cell factory, the biotechnology for “Compounds from Synechocystis” is still significantly lagging behind those for heterotrophic microbes (e.g., Escherichia coli). MDPI 2013-08-13 /pmc/articles/PMC3766872/ /pubmed/23945601 http://dx.doi.org/10.3390/md11082894 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Yu, Yi
You, Le
Liu, Dianyi
Hollinshead, Whitney
Tang, Yinjie J.
Zhang, Fuzhong
Development of Synechocystis sp. PCC 6803 as a Phototrophic Cell Factory
title Development of Synechocystis sp. PCC 6803 as a Phototrophic Cell Factory
title_full Development of Synechocystis sp. PCC 6803 as a Phototrophic Cell Factory
title_fullStr Development of Synechocystis sp. PCC 6803 as a Phototrophic Cell Factory
title_full_unstemmed Development of Synechocystis sp. PCC 6803 as a Phototrophic Cell Factory
title_short Development of Synechocystis sp. PCC 6803 as a Phototrophic Cell Factory
title_sort development of synechocystis sp. pcc 6803 as a phototrophic cell factory
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766872/
https://www.ncbi.nlm.nih.gov/pubmed/23945601
http://dx.doi.org/10.3390/md11082894
work_keys_str_mv AT yuyi developmentofsynechocystissppcc6803asaphototrophiccellfactory
AT youle developmentofsynechocystissppcc6803asaphototrophiccellfactory
AT liudianyi developmentofsynechocystissppcc6803asaphototrophiccellfactory
AT hollinsheadwhitney developmentofsynechocystissppcc6803asaphototrophiccellfactory
AT tangyinjiej developmentofsynechocystissppcc6803asaphototrophiccellfactory
AT zhangfuzhong developmentofsynechocystissppcc6803asaphototrophiccellfactory