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The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility

Because of the wide use of biodegradable materials in tissue engineering, it is necessary to obtain biocompatible polymers with different mechanical and physical properties as well as degradation ratio. Novel co- and terpolymers of various composition and chain microstructure have been developed and...

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Autores principales: Orchel, Arkadiusz, Jelonek, Katarzyna, Kasperczyk, Janusz, Dobrzynski, Piotr, Marcinkowski, Andrzej, Pamula, Elzbieta, Orchel, Joanna, Bielecki, Ireneusz, Kulczycka, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766997/
https://www.ncbi.nlm.nih.gov/pubmed/24062998
http://dx.doi.org/10.1155/2013/176946
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author Orchel, Arkadiusz
Jelonek, Katarzyna
Kasperczyk, Janusz
Dobrzynski, Piotr
Marcinkowski, Andrzej
Pamula, Elzbieta
Orchel, Joanna
Bielecki, Ireneusz
Kulczycka, Anna
author_facet Orchel, Arkadiusz
Jelonek, Katarzyna
Kasperczyk, Janusz
Dobrzynski, Piotr
Marcinkowski, Andrzej
Pamula, Elzbieta
Orchel, Joanna
Bielecki, Ireneusz
Kulczycka, Anna
author_sort Orchel, Arkadiusz
collection PubMed
description Because of the wide use of biodegradable materials in tissue engineering, it is necessary to obtain biocompatible polymers with different mechanical and physical properties as well as degradation ratio. Novel co- and terpolymers of various composition and chain microstructure have been developed and applied for cell culture. The aim of this study was to evaluate the adhesion and proliferation of human chondrocytes to four biodegradable copolymers: lactide-coglycolide, lactide-co-ε-caprolactone, lactide-co-trimethylene carbonate, glycolide-co-ε-caprolactone, and one terpolymer glycolide-colactide-co-ε-caprolactone synthesized with the use of zirconium acetylacetonate as a nontoxic initiator. Chain microstructure of the copolymers was analyzed by means of (1)H and (13)C NMR spectroscopy and surface properties by AFM technique. Cell adhesion and proliferation were determined by CyQUANT Cell Proliferation Assay Kit. After 4 h the chondrocyte adhesion on the surface of studied materials was comparable to standard TCPS. Cell proliferation occurred on all the substrates; however, among the studied polymers poly(L-lactide-coglycolide) 85 : 15 that characterized the most blocky structure best supported cell growth. Chondrocytes retained the cell membrane integrity evaluated by the LDH release assay. As can be summarized from the results of the study, all the studied polymers are well tolerated by the cells that make them appropriate for human chondrocytes growth.
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spelling pubmed-37669972013-09-23 The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility Orchel, Arkadiusz Jelonek, Katarzyna Kasperczyk, Janusz Dobrzynski, Piotr Marcinkowski, Andrzej Pamula, Elzbieta Orchel, Joanna Bielecki, Ireneusz Kulczycka, Anna Biomed Res Int Research Article Because of the wide use of biodegradable materials in tissue engineering, it is necessary to obtain biocompatible polymers with different mechanical and physical properties as well as degradation ratio. Novel co- and terpolymers of various composition and chain microstructure have been developed and applied for cell culture. The aim of this study was to evaluate the adhesion and proliferation of human chondrocytes to four biodegradable copolymers: lactide-coglycolide, lactide-co-ε-caprolactone, lactide-co-trimethylene carbonate, glycolide-co-ε-caprolactone, and one terpolymer glycolide-colactide-co-ε-caprolactone synthesized with the use of zirconium acetylacetonate as a nontoxic initiator. Chain microstructure of the copolymers was analyzed by means of (1)H and (13)C NMR spectroscopy and surface properties by AFM technique. Cell adhesion and proliferation were determined by CyQUANT Cell Proliferation Assay Kit. After 4 h the chondrocyte adhesion on the surface of studied materials was comparable to standard TCPS. Cell proliferation occurred on all the substrates; however, among the studied polymers poly(L-lactide-coglycolide) 85 : 15 that characterized the most blocky structure best supported cell growth. Chondrocytes retained the cell membrane integrity evaluated by the LDH release assay. As can be summarized from the results of the study, all the studied polymers are well tolerated by the cells that make them appropriate for human chondrocytes growth. Hindawi Publishing Corporation 2013 2013-08-25 /pmc/articles/PMC3766997/ /pubmed/24062998 http://dx.doi.org/10.1155/2013/176946 Text en Copyright © 2013 Arkadiusz Orchel et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Orchel, Arkadiusz
Jelonek, Katarzyna
Kasperczyk, Janusz
Dobrzynski, Piotr
Marcinkowski, Andrzej
Pamula, Elzbieta
Orchel, Joanna
Bielecki, Ireneusz
Kulczycka, Anna
The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility
title The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility
title_full The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility
title_fullStr The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility
title_full_unstemmed The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility
title_short The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility
title_sort influence of chain microstructure of biodegradable copolyesters obtained with low-toxic zirconium initiator to in vitro biocompatibility
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766997/
https://www.ncbi.nlm.nih.gov/pubmed/24062998
http://dx.doi.org/10.1155/2013/176946
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