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The First Outbreak of Giardiasis with Drinking Water in Korea
OBJECTIVES: To identify the pathogen of the diarrhea outbreak in a village in Jeollabuk province in Korea in April 2010. METHODS: DNA extraction was performed from the 120 L of collected water, which was centrifuged at 10,000 x g for 30 min. PCR reactions were conducted in a total of 25 ul, which in...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korea Centers for Disease Control and Prevention
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767097/ https://www.ncbi.nlm.nih.gov/pubmed/24159537 http://dx.doi.org/10.1016/j.phrp.2013.03.003 |
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author | Cheun, Hyeng-Il Kim, Cheon-Hyeon Cho, Shin-Hyeong Ma, Da-Won Goo, Bo-La Na, Mun-Su Youn, Seung-Ki Lee, Won-Ja |
author_facet | Cheun, Hyeng-Il Kim, Cheon-Hyeon Cho, Shin-Hyeong Ma, Da-Won Goo, Bo-La Na, Mun-Su Youn, Seung-Ki Lee, Won-Ja |
author_sort | Cheun, Hyeng-Il |
collection | PubMed |
description | OBJECTIVES: To identify the pathogen of the diarrhea outbreak in a village in Jeollabuk province in Korea in April 2010. METHODS: DNA extraction was performed from the 120 L of collected water, which was centrifuged at 10,000 x g for 30 min. PCR reactions were conducted in a total of 25 ul, which included PCR premix (GenDEPOT, Barker, TX, USA), 2 ul (∼100 ng) of extracted DNA, and 10 pmol of each primer. RESULTS: Nine people out of 25 had a symptom of abdominal pain accompanied by diarrhea after they used stored valley water in a water tank as a provisional water supply source without chlorine sterilization. Among them Giardia lamblia was detected in fecal samples of 7 people using the polymerase chain reaction method. Although G. lamblia was also detected from water provided by the provisional water supply system stored in the water tank and used as drinking water, it was not detected in the water tank itself. This water-borne outbreak is considered to have occurred when the provisional water supply tube was destroyed under a building construction and contaminated by G. lamblia, but its precise cause has not been clarified. CONCLUSION: This outbreak resulting from G. lamblia is very meaningful as the first outbreak of an infection by a water-borne parasite in Korea. |
format | Online Article Text |
id | pubmed-3767097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Korea Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-37670972013-10-24 The First Outbreak of Giardiasis with Drinking Water in Korea Cheun, Hyeng-Il Kim, Cheon-Hyeon Cho, Shin-Hyeong Ma, Da-Won Goo, Bo-La Na, Mun-Su Youn, Seung-Ki Lee, Won-Ja Osong Public Health Res Perspect Original Article OBJECTIVES: To identify the pathogen of the diarrhea outbreak in a village in Jeollabuk province in Korea in April 2010. METHODS: DNA extraction was performed from the 120 L of collected water, which was centrifuged at 10,000 x g for 30 min. PCR reactions were conducted in a total of 25 ul, which included PCR premix (GenDEPOT, Barker, TX, USA), 2 ul (∼100 ng) of extracted DNA, and 10 pmol of each primer. RESULTS: Nine people out of 25 had a symptom of abdominal pain accompanied by diarrhea after they used stored valley water in a water tank as a provisional water supply source without chlorine sterilization. Among them Giardia lamblia was detected in fecal samples of 7 people using the polymerase chain reaction method. Although G. lamblia was also detected from water provided by the provisional water supply system stored in the water tank and used as drinking water, it was not detected in the water tank itself. This water-borne outbreak is considered to have occurred when the provisional water supply tube was destroyed under a building construction and contaminated by G. lamblia, but its precise cause has not been clarified. CONCLUSION: This outbreak resulting from G. lamblia is very meaningful as the first outbreak of an infection by a water-borne parasite in Korea. Korea Centers for Disease Control and Prevention 2013-04 /pmc/articles/PMC3767097/ /pubmed/24159537 http://dx.doi.org/10.1016/j.phrp.2013.03.003 Text en Copyright ©2013, Korea Centers for Disease Control and Prevention This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Cheun, Hyeng-Il Kim, Cheon-Hyeon Cho, Shin-Hyeong Ma, Da-Won Goo, Bo-La Na, Mun-Su Youn, Seung-Ki Lee, Won-Ja The First Outbreak of Giardiasis with Drinking Water in Korea |
title | The First Outbreak of Giardiasis with Drinking Water in Korea |
title_full | The First Outbreak of Giardiasis with Drinking Water in Korea |
title_fullStr | The First Outbreak of Giardiasis with Drinking Water in Korea |
title_full_unstemmed | The First Outbreak of Giardiasis with Drinking Water in Korea |
title_short | The First Outbreak of Giardiasis with Drinking Water in Korea |
title_sort | first outbreak of giardiasis with drinking water in korea |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767097/ https://www.ncbi.nlm.nih.gov/pubmed/24159537 http://dx.doi.org/10.1016/j.phrp.2013.03.003 |
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