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Angiotensin Type 1a Receptor Signaling Is Not Necessary for the Production of Reactive Oxygen Species in Polymorphonuclear Leukocytes

Background. Although angiotensin II (Ang II) has inflammatory effects, little is known about its role in polymorphonuclear leucocytes (PMLs). To elucidate the role of Ang II in PMLs ROS production, we examined hydrogen peroxide (H(2)O(2)), one of the ROS, and NO production in AT1a receptor knockout...

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Autores principales: Yamato, Fumiko, Takaya, Junji, Tsuji, Shoji, Hasui, Masafumi, Kaneko, Kazunari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scholarly Research Network 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767344/
https://www.ncbi.nlm.nih.gov/pubmed/24049645
http://dx.doi.org/10.5402/2012/347852
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author Yamato, Fumiko
Takaya, Junji
Tsuji, Shoji
Hasui, Masafumi
Kaneko, Kazunari
author_facet Yamato, Fumiko
Takaya, Junji
Tsuji, Shoji
Hasui, Masafumi
Kaneko, Kazunari
author_sort Yamato, Fumiko
collection PubMed
description Background. Although angiotensin II (Ang II) has inflammatory effects, little is known about its role in polymorphonuclear leucocytes (PMLs). To elucidate the role of Ang II in PMLs ROS production, we examined hydrogen peroxide (H(2)O(2)), one of the ROS, and NO production in AT1a receptor knockout (AT1KO) mice. Methods and Results. PMLs were analyzed from Ang II type 1a receptor knockout mice (AT1KO) and C57BL/6 wild type mice. Using flow cytometry, we studied hydrogen peroxide (H(2)O(2)) production from PMLs after Staphylococcus aureus phagocytosis or phorbol myristate acetate (PMA) stimulation. Nitric oxide (NO) production in the AT1KO was low at basal and after phagocytosis. In the AT1KO, basal H(2)O(2) production was low. After PMA or phagocytosis stimulation, however, H(2)O(2) production was comparable to wild type mice. Next we studied the H(2)O(2) production in C57BL/6 mice exposed to Ang II or saline. H(2)O(2) production stimulated by PMA or phagocytosis did not differ between the two groups. Conclusions. AT1a pathway is not necessary for PMLs H(2)O(2) production but for NO production. There was a compensatory pathway for H(2)O(2) production other than the AT1a receptor.
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spelling pubmed-37673442013-09-18 Angiotensin Type 1a Receptor Signaling Is Not Necessary for the Production of Reactive Oxygen Species in Polymorphonuclear Leukocytes Yamato, Fumiko Takaya, Junji Tsuji, Shoji Hasui, Masafumi Kaneko, Kazunari ISRN Inflamm Research Article Background. Although angiotensin II (Ang II) has inflammatory effects, little is known about its role in polymorphonuclear leucocytes (PMLs). To elucidate the role of Ang II in PMLs ROS production, we examined hydrogen peroxide (H(2)O(2)), one of the ROS, and NO production in AT1a receptor knockout (AT1KO) mice. Methods and Results. PMLs were analyzed from Ang II type 1a receptor knockout mice (AT1KO) and C57BL/6 wild type mice. Using flow cytometry, we studied hydrogen peroxide (H(2)O(2)) production from PMLs after Staphylococcus aureus phagocytosis or phorbol myristate acetate (PMA) stimulation. Nitric oxide (NO) production in the AT1KO was low at basal and after phagocytosis. In the AT1KO, basal H(2)O(2) production was low. After PMA or phagocytosis stimulation, however, H(2)O(2) production was comparable to wild type mice. Next we studied the H(2)O(2) production in C57BL/6 mice exposed to Ang II or saline. H(2)O(2) production stimulated by PMA or phagocytosis did not differ between the two groups. Conclusions. AT1a pathway is not necessary for PMLs H(2)O(2) production but for NO production. There was a compensatory pathway for H(2)O(2) production other than the AT1a receptor. International Scholarly Research Network 2012-12-04 /pmc/articles/PMC3767344/ /pubmed/24049645 http://dx.doi.org/10.5402/2012/347852 Text en Copyright © 2012 Fumiko Yamato et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yamato, Fumiko
Takaya, Junji
Tsuji, Shoji
Hasui, Masafumi
Kaneko, Kazunari
Angiotensin Type 1a Receptor Signaling Is Not Necessary for the Production of Reactive Oxygen Species in Polymorphonuclear Leukocytes
title Angiotensin Type 1a Receptor Signaling Is Not Necessary for the Production of Reactive Oxygen Species in Polymorphonuclear Leukocytes
title_full Angiotensin Type 1a Receptor Signaling Is Not Necessary for the Production of Reactive Oxygen Species in Polymorphonuclear Leukocytes
title_fullStr Angiotensin Type 1a Receptor Signaling Is Not Necessary for the Production of Reactive Oxygen Species in Polymorphonuclear Leukocytes
title_full_unstemmed Angiotensin Type 1a Receptor Signaling Is Not Necessary for the Production of Reactive Oxygen Species in Polymorphonuclear Leukocytes
title_short Angiotensin Type 1a Receptor Signaling Is Not Necessary for the Production of Reactive Oxygen Species in Polymorphonuclear Leukocytes
title_sort angiotensin type 1a receptor signaling is not necessary for the production of reactive oxygen species in polymorphonuclear leukocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767344/
https://www.ncbi.nlm.nih.gov/pubmed/24049645
http://dx.doi.org/10.5402/2012/347852
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