Cargando…

Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice

Among T3 receptors, TRα1 is ubiquitous and its deletion or a specific expression of a dominant-negative TRα1 isoform in Sertoli cell leads to an increase in testis weight and sperm production. The identification of a 43-kDa truncated form of the nuclear receptor TRα1 (p43) in the mitochondrial matri...

Descripción completa

Detalles Bibliográficos
Autores principales: Fumel, Betty, Roy, Stéphanie, Fouchécourt, Sophie, Livera, Gabriel, Parent, Anne-Simone, Casas, François, Guillou, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767600/
https://www.ncbi.nlm.nih.gov/pubmed/24040148
http://dx.doi.org/10.1371/journal.pone.0074015
_version_ 1782283670142320640
author Fumel, Betty
Roy, Stéphanie
Fouchécourt, Sophie
Livera, Gabriel
Parent, Anne-Simone
Casas, François
Guillou, Florian
author_facet Fumel, Betty
Roy, Stéphanie
Fouchécourt, Sophie
Livera, Gabriel
Parent, Anne-Simone
Casas, François
Guillou, Florian
author_sort Fumel, Betty
collection PubMed
description Among T3 receptors, TRα1 is ubiquitous and its deletion or a specific expression of a dominant-negative TRα1 isoform in Sertoli cell leads to an increase in testis weight and sperm production. The identification of a 43-kDa truncated form of the nuclear receptor TRα1 (p43) in the mitochondrial matrix led us to test the hypothesis that this mitochondrial transcription factor could regulate Sertoli cell proliferation. Here we report that p43 depletion in mice increases testis weight and sperm reserve. In addition, we found that p43 deletion increases Sertoli cell proliferation in postnatal testis at 3 days of development. Electron microscopy studies evidence an alteration of mitochondrial morphology observed specifically in Sertoli cells of p43−/− mice. Moreover, gene expression studies indicate that the lack of p43 in testis induced an alteration of the mitochondrial-nuclear cross-talk. In particular, the up-regulation of Cdk4 and c-myc pathway in p43−/− probably explain the extended proliferation recorded in Sertoli cells of these mice. Our finding suggests that T3 limits post-natal Sertoli cell proliferation mainly through its mitochondrial T3 receptor p43.
format Online
Article
Text
id pubmed-3767600
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-37676002013-09-13 Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice Fumel, Betty Roy, Stéphanie Fouchécourt, Sophie Livera, Gabriel Parent, Anne-Simone Casas, François Guillou, Florian PLoS One Research Article Among T3 receptors, TRα1 is ubiquitous and its deletion or a specific expression of a dominant-negative TRα1 isoform in Sertoli cell leads to an increase in testis weight and sperm production. The identification of a 43-kDa truncated form of the nuclear receptor TRα1 (p43) in the mitochondrial matrix led us to test the hypothesis that this mitochondrial transcription factor could regulate Sertoli cell proliferation. Here we report that p43 depletion in mice increases testis weight and sperm reserve. In addition, we found that p43 deletion increases Sertoli cell proliferation in postnatal testis at 3 days of development. Electron microscopy studies evidence an alteration of mitochondrial morphology observed specifically in Sertoli cells of p43−/− mice. Moreover, gene expression studies indicate that the lack of p43 in testis induced an alteration of the mitochondrial-nuclear cross-talk. In particular, the up-regulation of Cdk4 and c-myc pathway in p43−/− probably explain the extended proliferation recorded in Sertoli cells of these mice. Our finding suggests that T3 limits post-natal Sertoli cell proliferation mainly through its mitochondrial T3 receptor p43. Public Library of Science 2013-09-09 /pmc/articles/PMC3767600/ /pubmed/24040148 http://dx.doi.org/10.1371/journal.pone.0074015 Text en © 2013 Fumel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fumel, Betty
Roy, Stéphanie
Fouchécourt, Sophie
Livera, Gabriel
Parent, Anne-Simone
Casas, François
Guillou, Florian
Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice
title Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice
title_full Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice
title_fullStr Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice
title_full_unstemmed Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice
title_short Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice
title_sort depletion of the p43 mitochondrial t3 receptor increases sertoli cell proliferation in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767600/
https://www.ncbi.nlm.nih.gov/pubmed/24040148
http://dx.doi.org/10.1371/journal.pone.0074015
work_keys_str_mv AT fumelbetty depletionofthep43mitochondrialt3receptorincreasessertolicellproliferationinmice
AT roystephanie depletionofthep43mitochondrialt3receptorincreasessertolicellproliferationinmice
AT fouchecourtsophie depletionofthep43mitochondrialt3receptorincreasessertolicellproliferationinmice
AT liveragabriel depletionofthep43mitochondrialt3receptorincreasessertolicellproliferationinmice
AT parentannesimone depletionofthep43mitochondrialt3receptorincreasessertolicellproliferationinmice
AT casasfrancois depletionofthep43mitochondrialt3receptorincreasessertolicellproliferationinmice
AT guillouflorian depletionofthep43mitochondrialt3receptorincreasessertolicellproliferationinmice