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Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice
Among T3 receptors, TRα1 is ubiquitous and its deletion or a specific expression of a dominant-negative TRα1 isoform in Sertoli cell leads to an increase in testis weight and sperm production. The identification of a 43-kDa truncated form of the nuclear receptor TRα1 (p43) in the mitochondrial matri...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767600/ https://www.ncbi.nlm.nih.gov/pubmed/24040148 http://dx.doi.org/10.1371/journal.pone.0074015 |
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author | Fumel, Betty Roy, Stéphanie Fouchécourt, Sophie Livera, Gabriel Parent, Anne-Simone Casas, François Guillou, Florian |
author_facet | Fumel, Betty Roy, Stéphanie Fouchécourt, Sophie Livera, Gabriel Parent, Anne-Simone Casas, François Guillou, Florian |
author_sort | Fumel, Betty |
collection | PubMed |
description | Among T3 receptors, TRα1 is ubiquitous and its deletion or a specific expression of a dominant-negative TRα1 isoform in Sertoli cell leads to an increase in testis weight and sperm production. The identification of a 43-kDa truncated form of the nuclear receptor TRα1 (p43) in the mitochondrial matrix led us to test the hypothesis that this mitochondrial transcription factor could regulate Sertoli cell proliferation. Here we report that p43 depletion in mice increases testis weight and sperm reserve. In addition, we found that p43 deletion increases Sertoli cell proliferation in postnatal testis at 3 days of development. Electron microscopy studies evidence an alteration of mitochondrial morphology observed specifically in Sertoli cells of p43−/− mice. Moreover, gene expression studies indicate that the lack of p43 in testis induced an alteration of the mitochondrial-nuclear cross-talk. In particular, the up-regulation of Cdk4 and c-myc pathway in p43−/− probably explain the extended proliferation recorded in Sertoli cells of these mice. Our finding suggests that T3 limits post-natal Sertoli cell proliferation mainly through its mitochondrial T3 receptor p43. |
format | Online Article Text |
id | pubmed-3767600 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37676002013-09-13 Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice Fumel, Betty Roy, Stéphanie Fouchécourt, Sophie Livera, Gabriel Parent, Anne-Simone Casas, François Guillou, Florian PLoS One Research Article Among T3 receptors, TRα1 is ubiquitous and its deletion or a specific expression of a dominant-negative TRα1 isoform in Sertoli cell leads to an increase in testis weight and sperm production. The identification of a 43-kDa truncated form of the nuclear receptor TRα1 (p43) in the mitochondrial matrix led us to test the hypothesis that this mitochondrial transcription factor could regulate Sertoli cell proliferation. Here we report that p43 depletion in mice increases testis weight and sperm reserve. In addition, we found that p43 deletion increases Sertoli cell proliferation in postnatal testis at 3 days of development. Electron microscopy studies evidence an alteration of mitochondrial morphology observed specifically in Sertoli cells of p43−/− mice. Moreover, gene expression studies indicate that the lack of p43 in testis induced an alteration of the mitochondrial-nuclear cross-talk. In particular, the up-regulation of Cdk4 and c-myc pathway in p43−/− probably explain the extended proliferation recorded in Sertoli cells of these mice. Our finding suggests that T3 limits post-natal Sertoli cell proliferation mainly through its mitochondrial T3 receptor p43. Public Library of Science 2013-09-09 /pmc/articles/PMC3767600/ /pubmed/24040148 http://dx.doi.org/10.1371/journal.pone.0074015 Text en © 2013 Fumel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Fumel, Betty Roy, Stéphanie Fouchécourt, Sophie Livera, Gabriel Parent, Anne-Simone Casas, François Guillou, Florian Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice |
title | Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice |
title_full | Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice |
title_fullStr | Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice |
title_full_unstemmed | Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice |
title_short | Depletion of the p43 Mitochondrial T3 Receptor Increases Sertoli Cell Proliferation in Mice |
title_sort | depletion of the p43 mitochondrial t3 receptor increases sertoli cell proliferation in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767600/ https://www.ncbi.nlm.nih.gov/pubmed/24040148 http://dx.doi.org/10.1371/journal.pone.0074015 |
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