Cargando…
Translation of Atherosclerotic Plaque Phase-Contrast CT Imaging from Synchrotron Radiation to a Conventional Lab-Based X-Ray Source
OBJECTIVES: Phase-contrast imaging is a novel X-ray based technique that provides enhanced soft tissue contrast. The aim of this study was to evaluate the feasibility of visualizing human carotid arteries by grating-based phase-contrast tomography (PC-CT) at two different experimental set-ups: (i) a...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767700/ https://www.ncbi.nlm.nih.gov/pubmed/24039969 http://dx.doi.org/10.1371/journal.pone.0073513 |
_version_ | 1782283692701384704 |
---|---|
author | Saam, Tobias Herzen, Julia Hetterich, Holger Fill, Sandra Willner, Marian Stockmar, Marco Achterhold, Klaus Zanette, Irene Weitkamp, Timm Schüller, Ulrich Auweter, Sigrid Adam-Neumair, Silvia Nikolaou, Konstantin Reiser, Maximilian F. Pfeiffer, Franz Bamberg, Fabian |
author_facet | Saam, Tobias Herzen, Julia Hetterich, Holger Fill, Sandra Willner, Marian Stockmar, Marco Achterhold, Klaus Zanette, Irene Weitkamp, Timm Schüller, Ulrich Auweter, Sigrid Adam-Neumair, Silvia Nikolaou, Konstantin Reiser, Maximilian F. Pfeiffer, Franz Bamberg, Fabian |
author_sort | Saam, Tobias |
collection | PubMed |
description | OBJECTIVES: Phase-contrast imaging is a novel X-ray based technique that provides enhanced soft tissue contrast. The aim of this study was to evaluate the feasibility of visualizing human carotid arteries by grating-based phase-contrast tomography (PC-CT) at two different experimental set-ups: (i) applying synchrotron radiation and (ii) using a conventional X-ray tube. MATERIALS AND METHODS: Five ex-vivo carotid artery specimens were examined with PC-CT either at the European Synchrotron Radiation Facility using a monochromatic X-ray beam (2 specimens; 23 keV; pixel size 5.4 µm), or at a laboratory set-up on a conventional X-ray tube (3 specimens; 35-40 kVp; 70 mA; pixel size 100 µm). Tomographic images were reconstructed and compared to histopathology. Two independent readers determined vessel dimensions and one reader determined signal-to-noise ratios (SNR) between PC-CT and absorption images. RESULTS: In total, 51 sections were included in the analysis. Images from both set-ups provided sufficient contrast to differentiate individual vessel layers. All PCI-based measurements strongly predicted but significantly overestimated lumen, intima and vessel wall area for both the synchrotron and the laboratory-based measurements as compared with histology (all p<0.001 with slope >0.53 per mm(2), 95%-CI: 0.35 to 0.70). Although synchrotron-based images were characterized by higher SNRs than laboratory-based images; both PC-CT set-ups had superior SNRs compared to corresponding conventional absorption-based images (p<0.001). Inter-reader reproducibility was excellent (ICCs >0.98 and >0.84 for synchrotron and for laboratory-based measurements; respectively). CONCLUSION: Experimental PC-CT of carotid specimens is feasible with both synchrotron and conventional X-ray sources, producing high-resolution images suitable for vessel characterization and atherosclerosis research. |
format | Online Article Text |
id | pubmed-3767700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37677002013-09-13 Translation of Atherosclerotic Plaque Phase-Contrast CT Imaging from Synchrotron Radiation to a Conventional Lab-Based X-Ray Source Saam, Tobias Herzen, Julia Hetterich, Holger Fill, Sandra Willner, Marian Stockmar, Marco Achterhold, Klaus Zanette, Irene Weitkamp, Timm Schüller, Ulrich Auweter, Sigrid Adam-Neumair, Silvia Nikolaou, Konstantin Reiser, Maximilian F. Pfeiffer, Franz Bamberg, Fabian PLoS One Research Article OBJECTIVES: Phase-contrast imaging is a novel X-ray based technique that provides enhanced soft tissue contrast. The aim of this study was to evaluate the feasibility of visualizing human carotid arteries by grating-based phase-contrast tomography (PC-CT) at two different experimental set-ups: (i) applying synchrotron radiation and (ii) using a conventional X-ray tube. MATERIALS AND METHODS: Five ex-vivo carotid artery specimens were examined with PC-CT either at the European Synchrotron Radiation Facility using a monochromatic X-ray beam (2 specimens; 23 keV; pixel size 5.4 µm), or at a laboratory set-up on a conventional X-ray tube (3 specimens; 35-40 kVp; 70 mA; pixel size 100 µm). Tomographic images were reconstructed and compared to histopathology. Two independent readers determined vessel dimensions and one reader determined signal-to-noise ratios (SNR) between PC-CT and absorption images. RESULTS: In total, 51 sections were included in the analysis. Images from both set-ups provided sufficient contrast to differentiate individual vessel layers. All PCI-based measurements strongly predicted but significantly overestimated lumen, intima and vessel wall area for both the synchrotron and the laboratory-based measurements as compared with histology (all p<0.001 with slope >0.53 per mm(2), 95%-CI: 0.35 to 0.70). Although synchrotron-based images were characterized by higher SNRs than laboratory-based images; both PC-CT set-ups had superior SNRs compared to corresponding conventional absorption-based images (p<0.001). Inter-reader reproducibility was excellent (ICCs >0.98 and >0.84 for synchrotron and for laboratory-based measurements; respectively). CONCLUSION: Experimental PC-CT of carotid specimens is feasible with both synchrotron and conventional X-ray sources, producing high-resolution images suitable for vessel characterization and atherosclerosis research. Public Library of Science 2013-09-09 /pmc/articles/PMC3767700/ /pubmed/24039969 http://dx.doi.org/10.1371/journal.pone.0073513 Text en © 2013 Saam et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Saam, Tobias Herzen, Julia Hetterich, Holger Fill, Sandra Willner, Marian Stockmar, Marco Achterhold, Klaus Zanette, Irene Weitkamp, Timm Schüller, Ulrich Auweter, Sigrid Adam-Neumair, Silvia Nikolaou, Konstantin Reiser, Maximilian F. Pfeiffer, Franz Bamberg, Fabian Translation of Atherosclerotic Plaque Phase-Contrast CT Imaging from Synchrotron Radiation to a Conventional Lab-Based X-Ray Source |
title | Translation of Atherosclerotic Plaque Phase-Contrast CT Imaging from Synchrotron Radiation to a Conventional Lab-Based X-Ray Source |
title_full | Translation of Atherosclerotic Plaque Phase-Contrast CT Imaging from Synchrotron Radiation to a Conventional Lab-Based X-Ray Source |
title_fullStr | Translation of Atherosclerotic Plaque Phase-Contrast CT Imaging from Synchrotron Radiation to a Conventional Lab-Based X-Ray Source |
title_full_unstemmed | Translation of Atherosclerotic Plaque Phase-Contrast CT Imaging from Synchrotron Radiation to a Conventional Lab-Based X-Ray Source |
title_short | Translation of Atherosclerotic Plaque Phase-Contrast CT Imaging from Synchrotron Radiation to a Conventional Lab-Based X-Ray Source |
title_sort | translation of atherosclerotic plaque phase-contrast ct imaging from synchrotron radiation to a conventional lab-based x-ray source |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3767700/ https://www.ncbi.nlm.nih.gov/pubmed/24039969 http://dx.doi.org/10.1371/journal.pone.0073513 |
work_keys_str_mv | AT saamtobias translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT herzenjulia translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT hetterichholger translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT fillsandra translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT willnermarian translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT stockmarmarco translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT achterholdklaus translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT zanetteirene translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT weitkamptimm translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT schullerulrich translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT auwetersigrid translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT adamneumairsilvia translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT nikolaoukonstantin translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT reisermaximilianf translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT pfeifferfranz translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource AT bambergfabian translationofatheroscleroticplaquephasecontrastctimagingfromsynchrotronradiationtoaconventionallabbasedxraysource |