Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression

The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, incl...

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Detalles Bibliográficos
Autores principales: Góes, Luiz Gustavo Bentim, de Freitas, Antonio Carlos, Ferraz, Oilita Pereira, Rieger, Tania Tassinari, dos Santos, José Ferreira, Pereira, Alexandre, Beçak, Willy, Lindsey, Charles J., de Cassia Stocco, Rita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2008
Materias:
Medical Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768373/
https://www.ncbi.nlm.nih.gov/pubmed/24031166
http://dx.doi.org/10.1590/S1517-83822008000100001
Descripción
Sumario:The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression.

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