Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression

The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, incl...

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Autores principales: Góes, Luiz Gustavo Bentim, de Freitas, Antonio Carlos, Ferraz, Oilita Pereira, Rieger, Tania Tassinari, dos Santos, José Ferreira, Pereira, Alexandre, Beçak, Willy, Lindsey, Charles J., de Cassia Stocco, Rita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2008
Materias:
Medical Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768373/
https://www.ncbi.nlm.nih.gov/pubmed/24031166
http://dx.doi.org/10.1590/S1517-83822008000100001
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author Góes, Luiz Gustavo Bentim
de Freitas, Antonio Carlos
Ferraz, Oilita Pereira
Rieger, Tania Tassinari
dos Santos, José Ferreira
Pereira, Alexandre
Beçak, Willy
Lindsey, Charles J.
de Cassia Stocco, Rita
author_facet Góes, Luiz Gustavo Bentim
de Freitas, Antonio Carlos
Ferraz, Oilita Pereira
Rieger, Tania Tassinari
dos Santos, José Ferreira
Pereira, Alexandre
Beçak, Willy
Lindsey, Charles J.
de Cassia Stocco, Rita
author_sort Góes, Luiz Gustavo Bentim
collection PubMed
description The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression.
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spelling pubmed-37683732013-09-12 Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression Góes, Luiz Gustavo Bentim de Freitas, Antonio Carlos Ferraz, Oilita Pereira Rieger, Tania Tassinari dos Santos, José Ferreira Pereira, Alexandre Beçak, Willy Lindsey, Charles J. de Cassia Stocco, Rita Braz J Microbiol Medical Microbiology The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression. Sociedade Brasileira de Microbiologia 2008 2008-03-01 /pmc/articles/PMC3768373/ /pubmed/24031166 http://dx.doi.org/10.1590/S1517-83822008000100001 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical Microbiology
Góes, Luiz Gustavo Bentim
de Freitas, Antonio Carlos
Ferraz, Oilita Pereira
Rieger, Tania Tassinari
dos Santos, José Ferreira
Pereira, Alexandre
Beçak, Willy
Lindsey, Charles J.
de Cassia Stocco, Rita
Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression
title Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression
title_full Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression
title_fullStr Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression
title_full_unstemmed Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression
title_short Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression
title_sort bovine papillomavirus type 4 l1 gene transfection in a drosophila s2 cell expression system: absence of l1 protein expression
topic Medical Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768373/
https://www.ncbi.nlm.nih.gov/pubmed/24031166
http://dx.doi.org/10.1590/S1517-83822008000100001
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