Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance

Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates,...

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Autores principales: de Souza Moraes, Paulo Ricardo, Chimara, Erica, Telles, Maria Alice da Silva, Ueki, Suely Yoko Misuka, Cunha, Eunice Atsuko Totumi, Honer, Michael Robin, Leão, Sylvia Cardoso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2008
Materias:
Medical Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768383/
https://www.ncbi.nlm.nih.gov/pubmed/24031214
http://dx.doi.org/10.1590/S1517-838220080002000013
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author de Souza Moraes, Paulo Ricardo
Chimara, Erica
Telles, Maria Alice da Silva
Ueki, Suely Yoko Misuka
Cunha, Eunice Atsuko Totumi
Honer, Michael Robin
Leão, Sylvia Cardoso
author_facet de Souza Moraes, Paulo Ricardo
Chimara, Erica
Telles, Maria Alice da Silva
Ueki, Suely Yoko Misuka
Cunha, Eunice Atsuko Totumi
Honer, Michael Robin
Leão, Sylvia Cardoso
author_sort de Souza Moraes, Paulo Ricardo
collection PubMed
description Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates.
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spelling pubmed-37683832013-09-12 Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance de Souza Moraes, Paulo Ricardo Chimara, Erica Telles, Maria Alice da Silva Ueki, Suely Yoko Misuka Cunha, Eunice Atsuko Totumi Honer, Michael Robin Leão, Sylvia Cardoso Braz J Microbiol Medical Microbiology Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates. Sociedade Brasileira de Microbiologia 2008 2008-06-01 /pmc/articles/PMC3768383/ /pubmed/24031214 http://dx.doi.org/10.1590/S1517-838220080002000013 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical Microbiology
de Souza Moraes, Paulo Ricardo
Chimara, Erica
Telles, Maria Alice da Silva
Ueki, Suely Yoko Misuka
Cunha, Eunice Atsuko Totumi
Honer, Michael Robin
Leão, Sylvia Cardoso
Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance
title Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance
title_full Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance
title_fullStr Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance
title_full_unstemmed Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance
title_short Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance
title_sort identification of non-tuberculous mycobacteria from the central public health laboratory from mato grosso do sul and analysis of clinical relevance
topic Medical Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768383/
https://www.ncbi.nlm.nih.gov/pubmed/24031214
http://dx.doi.org/10.1590/S1517-838220080002000013
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