Comparison of three decontamination methods for Mycobacterium bovis isolation

Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological salin...

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Detalles Bibliográficos
Autores principales: Ambrosio, Simone Rodrigues, de Deus Oliveira, Eugenia Márcia, Rodriguez, Cesar Alejandro Rosales, Ferreira Neto, José Soares, Amaku, Marcos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2008
Materias:
Medical Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768399/
https://www.ncbi.nlm.nih.gov/pubmed/24031209
http://dx.doi.org/10.1590/S1517-83822008000200008
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author Ambrosio, Simone Rodrigues
de Deus Oliveira, Eugenia Márcia
Rodriguez, Cesar Alejandro Rosales
Ferreira Neto, José Soares
Amaku, Marcos
author_facet Ambrosio, Simone Rodrigues
de Deus Oliveira, Eugenia Márcia
Rodriguez, Cesar Alejandro Rosales
Ferreira Neto, José Soares
Amaku, Marcos
author_sort Ambrosio, Simone Rodrigues
collection PubMed
description Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.
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spelling pubmed-37683992013-09-12 Comparison of three decontamination methods for Mycobacterium bovis isolation Ambrosio, Simone Rodrigues de Deus Oliveira, Eugenia Márcia Rodriguez, Cesar Alejandro Rosales Ferreira Neto, José Soares Amaku, Marcos Braz J Microbiol Medical Microbiology Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method. Sociedade Brasileira de Microbiologia 2008 2008-06-01 /pmc/articles/PMC3768399/ /pubmed/24031209 http://dx.doi.org/10.1590/S1517-83822008000200008 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical Microbiology
Ambrosio, Simone Rodrigues
de Deus Oliveira, Eugenia Márcia
Rodriguez, Cesar Alejandro Rosales
Ferreira Neto, José Soares
Amaku, Marcos
Comparison of three decontamination methods for Mycobacterium bovis isolation
title Comparison of three decontamination methods for Mycobacterium bovis isolation
title_full Comparison of three decontamination methods for Mycobacterium bovis isolation
title_fullStr Comparison of three decontamination methods for Mycobacterium bovis isolation
title_full_unstemmed Comparison of three decontamination methods for Mycobacterium bovis isolation
title_short Comparison of three decontamination methods for Mycobacterium bovis isolation
title_sort comparison of three decontamination methods for mycobacterium bovis isolation
topic Medical Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768399/
https://www.ncbi.nlm.nih.gov/pubmed/24031209
http://dx.doi.org/10.1590/S1517-83822008000200008
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