Cargando…

Microbiological and histopathological aspects of canine pyometra

As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health....

Descripción completa

Detalles Bibliográficos
Autores principales: Coggan, Jennifer Anne, Melville, Priscilla Anne, de Oliveira, Clair Motos, Faustino, Marcelo, Moreno, Andréa Micke, Benites, Nilson Roberti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768417/
https://www.ncbi.nlm.nih.gov/pubmed/24031249
http://dx.doi.org/10.1590/S1517-838220080003000012
_version_ 1782283778523136000
author Coggan, Jennifer Anne
Melville, Priscilla Anne
de Oliveira, Clair Motos
Faustino, Marcelo
Moreno, Andréa Micke
Benites, Nilson Roberti
author_facet Coggan, Jennifer Anne
Melville, Priscilla Anne
de Oliveira, Clair Motos
Faustino, Marcelo
Moreno, Andréa Micke
Benites, Nilson Roberti
author_sort Coggan, Jennifer Anne
collection PubMed
description As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health. The microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the E. coli strains were identified using PCR method. This study also consisted of the counting of microorganisms colonies forming units in samples of intrauterine content, tests of antimicrobial susceptibility of the E. coli isolates and the histological examination of the uterus. E. coli was the most prevalent microorganism isolated (76.6%) and 120 strains (79.5%) were positive for sfa, 86 (56.9%) were positive for cnf, 87 (57.6%) were positive for pap, 52 (34.4%) were positive for hly, 51 (33.8%) were positive for iuc and 5 (3.3%) were positive for afa genes. One observed more sensitivity of E. coli to norfloxacin, polimixin B, sulphazotrin, chloranfenicol and enrofloxacin. In 42% of the samples of uterine walls where microorganisms were isolated, the sizes of the areas of the inflammatory responses corresponded to 39–56%. Virulence factors were identified in 98.0% of the strains evaluated, demonstrating a high frequency of potentially pathogenic E. coli. It must be considered that dogs are animals that are living in close proximity to man for thousands of years and have an important role in the transmission of E. coli to other animals and to man.
format Online
Article
Text
id pubmed-3768417
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-37684172013-09-12 Microbiological and histopathological aspects of canine pyometra Coggan, Jennifer Anne Melville, Priscilla Anne de Oliveira, Clair Motos Faustino, Marcelo Moreno, Andréa Micke Benites, Nilson Roberti Braz J Microbiol Veterinary Microbiology As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health. The microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the E. coli strains were identified using PCR method. This study also consisted of the counting of microorganisms colonies forming units in samples of intrauterine content, tests of antimicrobial susceptibility of the E. coli isolates and the histological examination of the uterus. E. coli was the most prevalent microorganism isolated (76.6%) and 120 strains (79.5%) were positive for sfa, 86 (56.9%) were positive for cnf, 87 (57.6%) were positive for pap, 52 (34.4%) were positive for hly, 51 (33.8%) were positive for iuc and 5 (3.3%) were positive for afa genes. One observed more sensitivity of E. coli to norfloxacin, polimixin B, sulphazotrin, chloranfenicol and enrofloxacin. In 42% of the samples of uterine walls where microorganisms were isolated, the sizes of the areas of the inflammatory responses corresponded to 39–56%. Virulence factors were identified in 98.0% of the strains evaluated, demonstrating a high frequency of potentially pathogenic E. coli. It must be considered that dogs are animals that are living in close proximity to man for thousands of years and have an important role in the transmission of E. coli to other animals and to man. Sociedade Brasileira de Microbiologia 2008 2008-09-01 /pmc/articles/PMC3768417/ /pubmed/24031249 http://dx.doi.org/10.1590/S1517-838220080003000012 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Veterinary Microbiology
Coggan, Jennifer Anne
Melville, Priscilla Anne
de Oliveira, Clair Motos
Faustino, Marcelo
Moreno, Andréa Micke
Benites, Nilson Roberti
Microbiological and histopathological aspects of canine pyometra
title Microbiological and histopathological aspects of canine pyometra
title_full Microbiological and histopathological aspects of canine pyometra
title_fullStr Microbiological and histopathological aspects of canine pyometra
title_full_unstemmed Microbiological and histopathological aspects of canine pyometra
title_short Microbiological and histopathological aspects of canine pyometra
title_sort microbiological and histopathological aspects of canine pyometra
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768417/
https://www.ncbi.nlm.nih.gov/pubmed/24031249
http://dx.doi.org/10.1590/S1517-838220080003000012
work_keys_str_mv AT cogganjenniferanne microbiologicalandhistopathologicalaspectsofcaninepyometra
AT melvillepriscillaanne microbiologicalandhistopathologicalaspectsofcaninepyometra
AT deoliveiraclairmotos microbiologicalandhistopathologicalaspectsofcaninepyometra
AT faustinomarcelo microbiologicalandhistopathologicalaspectsofcaninepyometra
AT morenoandreamicke microbiologicalandhistopathologicalaspectsofcaninepyometra
AT benitesnilsonroberti microbiologicalandhistopathologicalaspectsofcaninepyometra