An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum

ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for para...

Descripción completa

Detalles Bibliográficos
Autores principales: Marassi, Carla Dray, Silva, Marley, Oelemann, Walter Martin Roland, de Souza Fonseca, Leila, Ristow, Paula, Lilenbaum, Walter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2008
Materias:
Veterinary Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768432/
https://www.ncbi.nlm.nih.gov/pubmed/24031256
http://dx.doi.org/10.1590/S1517-838220080003000019
_version_ 1782283782572736512
author Marassi, Carla Dray
Silva, Marley
Oelemann, Walter Martin Roland
de Souza Fonseca, Leila
Ristow, Paula
Lilenbaum, Walter
author_facet Marassi, Carla Dray
Silva, Marley
Oelemann, Walter Martin Roland
de Souza Fonseca, Leila
Ristow, Paula
Lilenbaum, Walter
author_sort Marassi, Carla Dray
collection PubMed
description ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ > 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen.
format Online
Article
Text
id pubmed-3768432
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-37684322013-09-12 An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum Marassi, Carla Dray Silva, Marley Oelemann, Walter Martin Roland de Souza Fonseca, Leila Ristow, Paula Lilenbaum, Walter Braz J Microbiol Veterinary Microbiology ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ > 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen. Sociedade Brasileira de Microbiologia 2008 2008-09-01 /pmc/articles/PMC3768432/ /pubmed/24031256 http://dx.doi.org/10.1590/S1517-838220080003000019 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Veterinary Microbiology
Marassi, Carla Dray
Silva, Marley
Oelemann, Walter Martin Roland
de Souza Fonseca, Leila
Ristow, Paula
Lilenbaum, Walter
An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
title An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
title_full An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
title_fullStr An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
title_full_unstemmed An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
title_short An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
title_sort alternative for the preadsorption step in the paratuberculosis serodiagnosis: mycobacterium fortuitum
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768432/
https://www.ncbi.nlm.nih.gov/pubmed/24031256
http://dx.doi.org/10.1590/S1517-838220080003000019
work_keys_str_mv AT marassicarladray analternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum
AT silvamarley analternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum
AT oelemannwaltermartinroland analternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum
AT desouzafonsecaleila analternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum
AT ristowpaula analternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum
AT lilenbaumwalter analternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum
AT marassicarladray alternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum
AT silvamarley alternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum
AT oelemannwaltermartinroland alternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum
AT desouzafonsecaleila alternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum
AT ristowpaula alternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum
AT lilenbaumwalter alternativeforthepreadsorptionstepintheparatuberculosisserodiagnosismycobacteriumfortuitum

Ejemplares similares