Cargando…

Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR)

The study reports the occurrence of fumonisin producing Fusarium verticillioides in 90 samples of stored paddy (Oryza sativa L.) collected from different geographical regions of Karnataka, India. Fumonisin producing F. verticillioides was identified based on micromorphological characteristics and PC...

Descripción completa

Detalles Bibliográficos
Autores principales: Maheshwar, P.K., Moharram, S. Ahmed, Janardhana, G.R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768486/
https://www.ncbi.nlm.nih.gov/pubmed/24031332
http://dx.doi.org/10.1590/S1517-838220090001000023
_version_ 1782283797034696704
author Maheshwar, P.K.
Moharram, S. Ahmed
Janardhana, G.R.
author_facet Maheshwar, P.K.
Moharram, S. Ahmed
Janardhana, G.R.
author_sort Maheshwar, P.K.
collection PubMed
description The study reports the occurrence of fumonisin producing Fusarium verticillioides in 90 samples of stored paddy (Oryza sativa L.) collected from different geographical regions of Karnataka, India. Fumonisin producing F. verticillioides was identified based on micromorphological characteristics and PCR using two sets of primers. One set of primers was F. verticillioides species specific, which selectively amplified the intergenic space region of rDNA. The other set of primers was specific to fumonisin producing F. verticillioides. Eight paddy samples were positive for F. verticillioides. Eleven isolates obtained from these samples were capable of producing fumonisin.
format Online
Article
Text
id pubmed-3768486
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-37684862013-09-12 Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR) Maheshwar, P.K. Moharram, S. Ahmed Janardhana, G.R. Braz J Microbiol Food Microbiology The study reports the occurrence of fumonisin producing Fusarium verticillioides in 90 samples of stored paddy (Oryza sativa L.) collected from different geographical regions of Karnataka, India. Fumonisin producing F. verticillioides was identified based on micromorphological characteristics and PCR using two sets of primers. One set of primers was F. verticillioides species specific, which selectively amplified the intergenic space region of rDNA. The other set of primers was specific to fumonisin producing F. verticillioides. Eight paddy samples were positive for F. verticillioides. Eleven isolates obtained from these samples were capable of producing fumonisin. Sociedade Brasileira de Microbiologia 2009 2009-03-01 /pmc/articles/PMC3768486/ /pubmed/24031332 http://dx.doi.org/10.1590/S1517-838220090001000023 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Food Microbiology
Maheshwar, P.K.
Moharram, S. Ahmed
Janardhana, G.R.
Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR)
title Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR)
title_full Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR)
title_fullStr Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR)
title_full_unstemmed Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR)
title_short Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR)
title_sort detection of fumonisin producing fusarium verticillioides in paddy (oryza sativa l.) using polymerase chain reaction (pcr)
topic Food Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768486/
https://www.ncbi.nlm.nih.gov/pubmed/24031332
http://dx.doi.org/10.1590/S1517-838220090001000023
work_keys_str_mv AT maheshwarpk detectionoffumonisinproducingfusariumverticillioidesinpaddyoryzasativalusingpolymerasechainreactionpcr
AT moharramsahmed detectionoffumonisinproducingfusariumverticillioidesinpaddyoryzasativalusingpolymerasechainreactionpcr
AT janardhanagr detectionoffumonisinproducingfusariumverticillioidesinpaddyoryzasativalusingpolymerasechainreactionpcr