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Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source
Growth and enzymes production by Aspergillus flavipes FP-500 were evaluated on pectin, polygalacturonic acid, galacturonic acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial pH values. We found that the strain produced exopectinases, endopectinases and pectin lyases. Exopec...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sociedade Brasileira de Microbiologia
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768495/ https://www.ncbi.nlm.nih.gov/pubmed/24031315 http://dx.doi.org/10.1590/S1517-83822009000100006 |
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author | Martínez-Trujillo, Aurora Aranda, Juan S. Gómez-Sánchez, Carlos Trejo-Aguilar, Blanca Aguilar-Osorio, Guillermo |
author_facet | Martínez-Trujillo, Aurora Aranda, Juan S. Gómez-Sánchez, Carlos Trejo-Aguilar, Blanca Aguilar-Osorio, Guillermo |
author_sort | Martínez-Trujillo, Aurora |
collection | PubMed |
description | Growth and enzymes production by Aspergillus flavipes FP-500 were evaluated on pectin, polygalacturonic acid, galacturonic acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial pH values. We found that the strain produced exopectinases, endopectinases and pectin lyases. Exopectinases and pectin lyase were found to be produced at basal levels as constitutive enzymes and their production was modulated by the available carbon source and pH of culture medium and stimulated by the presence of inducer in the culture medium. Endo-pectinase was basically inducible and was only produced when pectin was used as carbon source. Our results suggest that pectinases in A. flavipes FP-500 are produced in a concerted way. The first enzyme to be produced was exopectinase followed by Pectin Lyase and Endo-pectinase. |
format | Online Article Text |
id | pubmed-3768495 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37684952013-09-12 Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source Martínez-Trujillo, Aurora Aranda, Juan S. Gómez-Sánchez, Carlos Trejo-Aguilar, Blanca Aguilar-Osorio, Guillermo Braz J Microbiol Industrial Microbiology Growth and enzymes production by Aspergillus flavipes FP-500 were evaluated on pectin, polygalacturonic acid, galacturonic acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial pH values. We found that the strain produced exopectinases, endopectinases and pectin lyases. Exopectinases and pectin lyase were found to be produced at basal levels as constitutive enzymes and their production was modulated by the available carbon source and pH of culture medium and stimulated by the presence of inducer in the culture medium. Endo-pectinase was basically inducible and was only produced when pectin was used as carbon source. Our results suggest that pectinases in A. flavipes FP-500 are produced in a concerted way. The first enzyme to be produced was exopectinase followed by Pectin Lyase and Endo-pectinase. Sociedade Brasileira de Microbiologia 2009 2009-03-01 /pmc/articles/PMC3768495/ /pubmed/24031315 http://dx.doi.org/10.1590/S1517-83822009000100006 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | Industrial Microbiology Martínez-Trujillo, Aurora Aranda, Juan S. Gómez-Sánchez, Carlos Trejo-Aguilar, Blanca Aguilar-Osorio, Guillermo Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source |
title | Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source |
title_full | Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source |
title_fullStr | Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source |
title_full_unstemmed | Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source |
title_short | Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source |
title_sort | constitutive and inducible pectinolytic enzymes from aspergillus flavipes fp-500 and their modulation by ph and carbon source |
topic | Industrial Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768495/ https://www.ncbi.nlm.nih.gov/pubmed/24031315 http://dx.doi.org/10.1590/S1517-83822009000100006 |
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