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Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration

Gamma linolenic acid (GLA, 18:3, cis- 6,9,12- octadecatrienoic acid), an important compound in n- 6 eicosanoid family biosynthesis, occurs in the lipids of a few plant and microbial sources. This study focused on the screening of microbial strains with suitable lipase activity for enrichment of GLA...

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Autores principales: Fregolente, Patricia B.L., Fregolente, Leonardo V., Maciel, Maria R.W., Carvalho, Patricia O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768573/
https://www.ncbi.nlm.nih.gov/pubmed/24031421
http://dx.doi.org/10.1590/S1517-83822009000400004
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author Fregolente, Patricia B.L.
Fregolente, Leonardo V.
Maciel, Maria R.W.
Carvalho, Patricia O.
author_facet Fregolente, Patricia B.L.
Fregolente, Leonardo V.
Maciel, Maria R.W.
Carvalho, Patricia O.
author_sort Fregolente, Patricia B.L.
collection PubMed
description Gamma linolenic acid (GLA, 18:3, cis- 6,9,12- octadecatrienoic acid), an important compound in n- 6 eicosanoid family biosynthesis, occurs in the lipids of a few plant and microbial sources. This study focused on the screening of microbial strains with suitable lipase activity for enrichment of GLA by selective hydrolysis of the borage oil (21.6 % of GLA/total fatty acids). Firstly, 352 microrganisms were tested for their lipolytic capacity using screening techniques on agar plates containing borage oil, strains were then selected and screened for their activity (U/mg) using both submerged fermentation (SmF) and solid state fermentation (SSF). The rate of hydrolysis and the selective preference of these hydrolytic enzymes towards fatty acids, with a special focus on enrichment of GLA were studied and compared with those obtained by two commercially-available lipases. Only one of the lipases tested during this study displayed selectivity, discriminating the GLA during the hydrolysis reaction. Using the enzymatic extract from Geotrichum candidum as a biocatalyst of the reaction, it was possible to obtain a percentage of 41.7% of GLA in acylglycerols fraction when the borage oil was treated in a fixed-bed reactor for 24 hours at 30ºC.
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spelling pubmed-37685732013-09-12 Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration Fregolente, Patricia B.L. Fregolente, Leonardo V. Maciel, Maria R.W. Carvalho, Patricia O. Braz J Microbiol Industrial Microbiology Gamma linolenic acid (GLA, 18:3, cis- 6,9,12- octadecatrienoic acid), an important compound in n- 6 eicosanoid family biosynthesis, occurs in the lipids of a few plant and microbial sources. This study focused on the screening of microbial strains with suitable lipase activity for enrichment of GLA by selective hydrolysis of the borage oil (21.6 % of GLA/total fatty acids). Firstly, 352 microrganisms were tested for their lipolytic capacity using screening techniques on agar plates containing borage oil, strains were then selected and screened for their activity (U/mg) using both submerged fermentation (SmF) and solid state fermentation (SSF). The rate of hydrolysis and the selective preference of these hydrolytic enzymes towards fatty acids, with a special focus on enrichment of GLA were studied and compared with those obtained by two commercially-available lipases. Only one of the lipases tested during this study displayed selectivity, discriminating the GLA during the hydrolysis reaction. Using the enzymatic extract from Geotrichum candidum as a biocatalyst of the reaction, it was possible to obtain a percentage of 41.7% of GLA in acylglycerols fraction when the borage oil was treated in a fixed-bed reactor for 24 hours at 30ºC. Sociedade Brasileira de Microbiologia 2009 2009-12-01 /pmc/articles/PMC3768573/ /pubmed/24031421 http://dx.doi.org/10.1590/S1517-83822009000400004 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Industrial Microbiology
Fregolente, Patricia B.L.
Fregolente, Leonardo V.
Maciel, Maria R.W.
Carvalho, Patricia O.
Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration
title Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration
title_full Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration
title_fullStr Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration
title_full_unstemmed Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration
title_short Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration
title_sort screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration
topic Industrial Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768573/
https://www.ncbi.nlm.nih.gov/pubmed/24031421
http://dx.doi.org/10.1590/S1517-83822009000400004
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