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Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera
The ethanol, methanol and acetone extracts of Aloe vera gel were studied for their antimicrobial activity against four Gram-positive and Gram-negative bacteria using agar well diffusion method. The extracts showed varied levels of antimicrobial activity against the tested pathogens. The ethanol and...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768575/ https://www.ncbi.nlm.nih.gov/pubmed/24031440 http://dx.doi.org/10.1590/S1517-838220090004000023 |
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author | Lawrence, Rubina Tripathi, Priyanka Jeyakumar, Ebenezer |
author_facet | Lawrence, Rubina Tripathi, Priyanka Jeyakumar, Ebenezer |
author_sort | Lawrence, Rubina |
collection | PubMed |
description | The ethanol, methanol and acetone extracts of Aloe vera gel were studied for their antimicrobial activity against four Gram-positive and Gram-negative bacteria using agar well diffusion method. The extracts showed varied levels of antimicrobial activity against the tested pathogens. The ethanol and methanol extracts showed higher activity while acetone extract, showed least or no activity against most of the tested pathogens. Fractions obtained from the extracts by Thin Layer and Column Chromatography were studied for their antagonistic properties using Spot Assay Technique. Compounds with maximum antibacterial activity isolated from the ethanol and methanol extracts were identified as p – coumaric acid (Mol. wt.165), ascorbic acid (Mol. wt.177 ), pyrocatechol (Mol. wt.110 ) and cinnamic acid (Mol. wt.148), on the basis of Gas Chromatography Mass Spectrometry. The study suggests the antimicrobial activity of the A. vera gel extract to be dependant on the synergistic effect of different compounds. With the broad spectral antimicrobial effect of A. vera gel, it could be further recommended in the treatment of various bacterial diseases. |
format | Online Article Text |
id | pubmed-3768575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37685752013-09-12 Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera Lawrence, Rubina Tripathi, Priyanka Jeyakumar, Ebenezer Braz J Microbiol General Microbiology The ethanol, methanol and acetone extracts of Aloe vera gel were studied for their antimicrobial activity against four Gram-positive and Gram-negative bacteria using agar well diffusion method. The extracts showed varied levels of antimicrobial activity against the tested pathogens. The ethanol and methanol extracts showed higher activity while acetone extract, showed least or no activity against most of the tested pathogens. Fractions obtained from the extracts by Thin Layer and Column Chromatography were studied for their antagonistic properties using Spot Assay Technique. Compounds with maximum antibacterial activity isolated from the ethanol and methanol extracts were identified as p – coumaric acid (Mol. wt.165), ascorbic acid (Mol. wt.177 ), pyrocatechol (Mol. wt.110 ) and cinnamic acid (Mol. wt.148), on the basis of Gas Chromatography Mass Spectrometry. The study suggests the antimicrobial activity of the A. vera gel extract to be dependant on the synergistic effect of different compounds. With the broad spectral antimicrobial effect of A. vera gel, it could be further recommended in the treatment of various bacterial diseases. Sociedade Brasileira de Microbiologia 2009 2009-12-01 /pmc/articles/PMC3768575/ /pubmed/24031440 http://dx.doi.org/10.1590/S1517-838220090004000023 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | General Microbiology Lawrence, Rubina Tripathi, Priyanka Jeyakumar, Ebenezer Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera |
title | Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera |
title_full | Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera |
title_fullStr | Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera |
title_full_unstemmed | Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera |
title_short | Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera |
title_sort | isolation, purification and evaluation of antibacterial agents from aloe vera |
topic | General Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768575/ https://www.ncbi.nlm.nih.gov/pubmed/24031440 http://dx.doi.org/10.1590/S1517-838220090004000023 |
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