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New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli

We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initia...

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Detalles Bibliográficos
Autores principales:	Xavier, Mauro Aparecido Souza, Kipnis, André, Torres, Fernando Araripe Gonçalves, Astofi-Filho, Spartaco
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Sociedade Brasileira de Microbiologia 2009
Materias:	Industrial Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768577/ https://www.ncbi.nlm.nih.gov/pubmed/24031424 http://dx.doi.org/10.1590/S1517-83822009000400007

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