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Concomitant production of protease and lipase by Bacillus Licheniformis VSG1: Production, purification and characterization

This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was...

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Detalles Bibliográficos
Autores principales: Sangeetha, R., Geetha, A., Arulpandi, I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768603/
https://www.ncbi.nlm.nih.gov/pubmed/24031479
http://dx.doi.org/10.1590/S1517-838220100001000026
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author Sangeetha, R.
Geetha, A.
Arulpandi, I.
author_facet Sangeetha, R.
Geetha, A.
Arulpandi, I.
author_sort Sangeetha, R.
collection PubMed
description This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and incubation time. The enzyme mixture comprising of both protease and lipase was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography to obtain 20-fold pure enzymes. The purified enzyme mixture was characterized to determine the optimum pH and temperature of protease and lipase, the response of the enzymes to inhibitors, additives and solvents. The molecular weight of both the enzymes was determined as 40 kDa on SDS-PAGE. The concomitant production of protease and lipase and the purification of both the enzymes in a single mixture have industrial significance, as many industrial processes use both protease and lipase together.
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spelling pubmed-37686032013-09-12 Concomitant production of protease and lipase by Bacillus Licheniformis VSG1: Production, purification and characterization Sangeetha, R. Geetha, A. Arulpandi, I. Braz J Microbiol Industrial Microbiology This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and incubation time. The enzyme mixture comprising of both protease and lipase was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography to obtain 20-fold pure enzymes. The purified enzyme mixture was characterized to determine the optimum pH and temperature of protease and lipase, the response of the enzymes to inhibitors, additives and solvents. The molecular weight of both the enzymes was determined as 40 kDa on SDS-PAGE. The concomitant production of protease and lipase and the purification of both the enzymes in a single mixture have industrial significance, as many industrial processes use both protease and lipase together. Sociedade Brasileira de Microbiologia 2010 2010-03-01 /pmc/articles/PMC3768603/ /pubmed/24031479 http://dx.doi.org/10.1590/S1517-838220100001000026 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Industrial Microbiology
Sangeetha, R.
Geetha, A.
Arulpandi, I.
Concomitant production of protease and lipase by Bacillus Licheniformis VSG1: Production, purification and characterization
title Concomitant production of protease and lipase by Bacillus Licheniformis VSG1: Production, purification and characterization
title_full Concomitant production of protease and lipase by Bacillus Licheniformis VSG1: Production, purification and characterization
title_fullStr Concomitant production of protease and lipase by Bacillus Licheniformis VSG1: Production, purification and characterization
title_full_unstemmed Concomitant production of protease and lipase by Bacillus Licheniformis VSG1: Production, purification and characterization
title_short Concomitant production of protease and lipase by Bacillus Licheniformis VSG1: Production, purification and characterization
title_sort concomitant production of protease and lipase by bacillus licheniformis vsg1: production, purification and characterization
topic Industrial Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768603/
https://www.ncbi.nlm.nih.gov/pubmed/24031479
http://dx.doi.org/10.1590/S1517-838220100001000026
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