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Photodynamic inactivation of four Candida species induced by photogem(®)

This study evaluated the in vitro susceptibility of C. albicans, C. dubliniensis, C. tropicalis and C. krusei to photodynamic therapy (PDT) induced by Photogem(®)and light emitted diode (LED). Suspensions of each Candida strain were treated with three photosensitizer (PS) concentrations (10, 25 and...

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Autores principales: Dovigo, Lívia Nordi, Pavarina, Ana Cláudia, Ribeiro, Daniela Garcia, Adriano, Cynthia Sanchez, Bagnato, Vanderlei Salvador
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768607/
https://www.ncbi.nlm.nih.gov/pubmed/24031462
http://dx.doi.org/10.1590/S1517-83822010000100009
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author Dovigo, Lívia Nordi
Pavarina, Ana Cláudia
Ribeiro, Daniela Garcia
Adriano, Cynthia Sanchez
Bagnato, Vanderlei Salvador
author_facet Dovigo, Lívia Nordi
Pavarina, Ana Cláudia
Ribeiro, Daniela Garcia
Adriano, Cynthia Sanchez
Bagnato, Vanderlei Salvador
author_sort Dovigo, Lívia Nordi
collection PubMed
description This study evaluated the in vitro susceptibility of C. albicans, C. dubliniensis, C. tropicalis and C. krusei to photodynamic therapy (PDT) induced by Photogem(®)and light emitted diode (LED). Suspensions of each Candida strain were treated with three photosensitizer (PS) concentrations (10, 25 and 50 mg/L) and exposed to 18, 25.5 and 37.5 J/cm(2) LED light fluences (λ ~ 455 nm). Control suspensions were treated only with PS concentrations, only exposed to the LED light fluences or not exposed to LED light or PS. Sixteen experimental conditions were obtained and each condition was repeated three times. From each sample, serial dilutions were obtained and aliquots were plated on Sabouraud Dextrose Agar. After incubation of plates (37 °C for 48 hours), colonies were counted (cfu/mL) and the data were statistically analyzed by ANOVA and the Tukey test (α=0.05). Complete killing of C. albicans was observed after 18 J/cm(2) in association with 50 mg/L of PS. C. dubliniensis were inactivated after 18 J/cm(2) using 25 mg/L of PS. The inactivation of C. tropicalis was observed after photosensitization with 25 mg/L and subsequent illumination at 25.5 J/cm(2). For C. krusei, none of the associations between PS and light resulted in complete killing of this species. PDT proved to be effective for the inactivation of C. albicans, C. dubliniensis and C. tropicalis. In addition, reduction in the viability of C. krusei was achieved with some of the PS and light associations.
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spelling pubmed-37686072013-09-12 Photodynamic inactivation of four Candida species induced by photogem(®) Dovigo, Lívia Nordi Pavarina, Ana Cláudia Ribeiro, Daniela Garcia Adriano, Cynthia Sanchez Bagnato, Vanderlei Salvador Braz J Microbiol Medical This study evaluated the in vitro susceptibility of C. albicans, C. dubliniensis, C. tropicalis and C. krusei to photodynamic therapy (PDT) induced by Photogem(®)and light emitted diode (LED). Suspensions of each Candida strain were treated with three photosensitizer (PS) concentrations (10, 25 and 50 mg/L) and exposed to 18, 25.5 and 37.5 J/cm(2) LED light fluences (λ ~ 455 nm). Control suspensions were treated only with PS concentrations, only exposed to the LED light fluences or not exposed to LED light or PS. Sixteen experimental conditions were obtained and each condition was repeated three times. From each sample, serial dilutions were obtained and aliquots were plated on Sabouraud Dextrose Agar. After incubation of plates (37 °C for 48 hours), colonies were counted (cfu/mL) and the data were statistically analyzed by ANOVA and the Tukey test (α=0.05). Complete killing of C. albicans was observed after 18 J/cm(2) in association with 50 mg/L of PS. C. dubliniensis were inactivated after 18 J/cm(2) using 25 mg/L of PS. The inactivation of C. tropicalis was observed after photosensitization with 25 mg/L and subsequent illumination at 25.5 J/cm(2). For C. krusei, none of the associations between PS and light resulted in complete killing of this species. PDT proved to be effective for the inactivation of C. albicans, C. dubliniensis and C. tropicalis. In addition, reduction in the viability of C. krusei was achieved with some of the PS and light associations. Sociedade Brasileira de Microbiologia 2010 2010-03-01 /pmc/articles/PMC3768607/ /pubmed/24031462 http://dx.doi.org/10.1590/S1517-83822010000100009 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical
Dovigo, Lívia Nordi
Pavarina, Ana Cláudia
Ribeiro, Daniela Garcia
Adriano, Cynthia Sanchez
Bagnato, Vanderlei Salvador
Photodynamic inactivation of four Candida species induced by photogem(®)
title Photodynamic inactivation of four Candida species induced by photogem(®)
title_full Photodynamic inactivation of four Candida species induced by photogem(®)
title_fullStr Photodynamic inactivation of four Candida species induced by photogem(®)
title_full_unstemmed Photodynamic inactivation of four Candida species induced by photogem(®)
title_short Photodynamic inactivation of four Candida species induced by photogem(®)
title_sort photodynamic inactivation of four candida species induced by photogem(®)
topic Medical
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768607/
https://www.ncbi.nlm.nih.gov/pubmed/24031462
http://dx.doi.org/10.1590/S1517-83822010000100009
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