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Isolation and fusion of protoplasts from the phytopathogenic fungus sclerotium rolfsii(sacc.)
Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5)/g mycelia and 2.8x1...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768609/ https://www.ncbi.nlm.nih.gov/pubmed/24031488 http://dx.doi.org/10.1590/S1517-838220100001000035 |
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author | Cai, Yun Christias, Christos |
author_facet | Cai, Yun Christias, Christos |
author_sort | Cai, Yun |
collection | PubMed |
description | Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5)/g mycelia and 2.8x10(5)/g mycelia were formed in strains A and R respectively. Osmotic stabilizer sucrose 1M gave maximum yield. Lysing enzyme at the rate of 15mg/ml was found best for yield. Fusion of protoplasts from strains A and R was carried out in fusion media containing PEG 4000 30% (w/v) with 0.2mM CaCl(2). Four fusants F1, F2, F3 and F4 were recovered. Morphological, physiological and pathogenic characters of fusants were compared with parent strains on carrots, beans and tomato. |
format | Online Article Text |
id | pubmed-3768609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37686092013-09-12 Isolation and fusion of protoplasts from the phytopathogenic fungus sclerotium rolfsii(sacc.) Cai, Yun Christias, Christos Braz J Microbiol General Microbiology Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5)/g mycelia and 2.8x10(5)/g mycelia were formed in strains A and R respectively. Osmotic stabilizer sucrose 1M gave maximum yield. Lysing enzyme at the rate of 15mg/ml was found best for yield. Fusion of protoplasts from strains A and R was carried out in fusion media containing PEG 4000 30% (w/v) with 0.2mM CaCl(2). Four fusants F1, F2, F3 and F4 were recovered. Morphological, physiological and pathogenic characters of fusants were compared with parent strains on carrots, beans and tomato. Sociedade Brasileira de Microbiologia 2010 2010-03-01 /pmc/articles/PMC3768609/ /pubmed/24031488 http://dx.doi.org/10.1590/S1517-838220100001000035 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | General Microbiology Cai, Yun Christias, Christos Isolation and fusion of protoplasts from the phytopathogenic fungus sclerotium rolfsii(sacc.) |
title | Isolation and fusion of protoplasts from the phytopathogenic fungus sclerotium rolfsii(sacc.) |
title_full | Isolation and fusion of protoplasts from the phytopathogenic fungus sclerotium rolfsii(sacc.) |
title_fullStr | Isolation and fusion of protoplasts from the phytopathogenic fungus sclerotium rolfsii(sacc.) |
title_full_unstemmed | Isolation and fusion of protoplasts from the phytopathogenic fungus sclerotium rolfsii(sacc.) |
title_short | Isolation and fusion of protoplasts from the phytopathogenic fungus sclerotium rolfsii(sacc.) |
title_sort | isolation and fusion of protoplasts from the phytopathogenic fungus sclerotium rolfsii(sacc.) |
topic | General Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768609/ https://www.ncbi.nlm.nih.gov/pubmed/24031488 http://dx.doi.org/10.1590/S1517-838220100001000035 |
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