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Phosphate Solubilization Potential and Phosphatase Activity Of Rhizospheric Trichoderma Spp.

Trichoderma sp., a well known biological control agent against several phytopathogens, was tested for its phosphate (P) solubilizing potential. Fourteen strains of Trichoderma sp. were isolated from the forest tree rhizospheres of pinus, deodar, bamboo, guava and oak on Trichoderma selective medium....

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Autores principales: Anil, Kapri, Lakshmi, Tewari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768635/
https://www.ncbi.nlm.nih.gov/pubmed/24031556
http://dx.doi.org/10.1590/S1517-83822010005000031
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author Anil, Kapri
Lakshmi, Tewari
author_facet Anil, Kapri
Lakshmi, Tewari
author_sort Anil, Kapri
collection PubMed
description Trichoderma sp., a well known biological control agent against several phytopathogens, was tested for its phosphate (P) solubilizing potential. Fourteen strains of Trichoderma sp. were isolated from the forest tree rhizospheres of pinus, deodar, bamboo, guava and oak on Trichoderma selective medium. The isolates were tested for their in-vitro P-solubilizing potential using National Botanical Research Institute Phosphate (NBRIP) broth containing tricalcium phosphate (TCP) as the sole P source, and compared with a standard culture of T. harzianum. All the cultures were found to solubilize TCP but with varying potential. The isolate DRT-1 showed maximum amount of soluble phosphate (404.07 εg.ml(-1)), followed by the standard culture of T. harzianum (386.42 εg.ml(-1)) after 96 h of incubation at 30±1(0)C. Extra-cellular acid and alkaline phosphatases of the fungus were induced only in the presence of insoluble phosphorus source (TCP). High extra-cellular alkaline phosphatase activity was recorded for the isolate DRT-1 (14.50 U.ml(-1)) followed by the standard culture (13.41 U.ml(-1)) at 72h. The cultures showed much lesser acid phosphatase activities. Under glasshouse conditions, Trichoderma sp. inoculation increased chickpea (Cicer arietinum) growth parameters including shoot length, root length, fresh and dry weight of shoot as well as roots, in P-deficient soil containing only bound phosphate (TCP). Shoot weight was increased by 23% and 33% by inoculation with the isolate DRT-1 in the soil amended with 100 and 200 mg TCP kg(-1) soil, respectively, after 60 d of sowing. The study explores high P-solubilizing potential of Trichoderma sp., which can be exploited for the solubilization of fixed phosphates present in the soil, thereby enhancing soil fertility and plant growth.
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spelling pubmed-37686352013-09-12 Phosphate Solubilization Potential and Phosphatase Activity Of Rhizospheric Trichoderma Spp. Anil, Kapri Lakshmi, Tewari Braz J Microbiol Microbial Physiology Trichoderma sp., a well known biological control agent against several phytopathogens, was tested for its phosphate (P) solubilizing potential. Fourteen strains of Trichoderma sp. were isolated from the forest tree rhizospheres of pinus, deodar, bamboo, guava and oak on Trichoderma selective medium. The isolates were tested for their in-vitro P-solubilizing potential using National Botanical Research Institute Phosphate (NBRIP) broth containing tricalcium phosphate (TCP) as the sole P source, and compared with a standard culture of T. harzianum. All the cultures were found to solubilize TCP but with varying potential. The isolate DRT-1 showed maximum amount of soluble phosphate (404.07 εg.ml(-1)), followed by the standard culture of T. harzianum (386.42 εg.ml(-1)) after 96 h of incubation at 30±1(0)C. Extra-cellular acid and alkaline phosphatases of the fungus were induced only in the presence of insoluble phosphorus source (TCP). High extra-cellular alkaline phosphatase activity was recorded for the isolate DRT-1 (14.50 U.ml(-1)) followed by the standard culture (13.41 U.ml(-1)) at 72h. The cultures showed much lesser acid phosphatase activities. Under glasshouse conditions, Trichoderma sp. inoculation increased chickpea (Cicer arietinum) growth parameters including shoot length, root length, fresh and dry weight of shoot as well as roots, in P-deficient soil containing only bound phosphate (TCP). Shoot weight was increased by 23% and 33% by inoculation with the isolate DRT-1 in the soil amended with 100 and 200 mg TCP kg(-1) soil, respectively, after 60 d of sowing. The study explores high P-solubilizing potential of Trichoderma sp., which can be exploited for the solubilization of fixed phosphates present in the soil, thereby enhancing soil fertility and plant growth. Sociedade Brasileira de Microbiologia 2010 2010-09-01 /pmc/articles/PMC3768635/ /pubmed/24031556 http://dx.doi.org/10.1590/S1517-83822010005000031 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ *** ***
spellingShingle Microbial Physiology
Anil, Kapri
Lakshmi, Tewari
Phosphate Solubilization Potential and Phosphatase Activity Of Rhizospheric Trichoderma Spp.
title Phosphate Solubilization Potential and Phosphatase Activity Of Rhizospheric Trichoderma Spp.
title_full Phosphate Solubilization Potential and Phosphatase Activity Of Rhizospheric Trichoderma Spp.
title_fullStr Phosphate Solubilization Potential and Phosphatase Activity Of Rhizospheric Trichoderma Spp.
title_full_unstemmed Phosphate Solubilization Potential and Phosphatase Activity Of Rhizospheric Trichoderma Spp.
title_short Phosphate Solubilization Potential and Phosphatase Activity Of Rhizospheric Trichoderma Spp.
title_sort phosphate solubilization potential and phosphatase activity of rhizospheric trichoderma spp.
topic Microbial Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768635/
https://www.ncbi.nlm.nih.gov/pubmed/24031556
http://dx.doi.org/10.1590/S1517-83822010005000031
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