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Rauvolfia Grandiflora (Apocynaceae) Extract Interferes With Staphylococcal Density, Enterotoxin Production And Antimicrobial Activity

Staphylococci bacteria are involved in many human and animal infections and development of alternative antimicrobial drugs against pathogenic bacteria is of great interest to the pharmaceutical industry. This study investigated the in vitro effect of Rauvolfia grandiflora methanol extract (root bark...

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Autores principales: de Almeida Carlos, Lanamar, da Silva Amaral, Kenas Aguiar, Curcino Vieira, Ivo José, Mathias, Leda, Braz-Filho, Raimundo, Silva Samarão, Solange, Vieira-da-Motta, Olney
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768640/
https://www.ncbi.nlm.nih.gov/pubmed/24031536
http://dx.doi.org/10.1590/S1517-83822010000300011
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author de Almeida Carlos, Lanamar
da Silva Amaral, Kenas Aguiar
Curcino Vieira, Ivo José
Mathias, Leda
Braz-Filho, Raimundo
Silva Samarão, Solange
Vieira-da-Motta, Olney
author_facet de Almeida Carlos, Lanamar
da Silva Amaral, Kenas Aguiar
Curcino Vieira, Ivo José
Mathias, Leda
Braz-Filho, Raimundo
Silva Samarão, Solange
Vieira-da-Motta, Olney
author_sort de Almeida Carlos, Lanamar
collection PubMed
description Staphylococci bacteria are involved in many human and animal infections and development of alternative antimicrobial drugs against pathogenic bacteria is of great interest to the pharmaceutical industry. This study investigated the in vitro effect of Rauvolfia grandiflora methanol extract (root bark fraction) (RGE) on the density of ATCC strains of Staphylococcus aureus and Staphylococcus epidermidis, and a clinical enterotoxin-producer, S. aureus bovine strain. The alkaloid, isoreserpiline, obtained from dichloromethane extract of R. grandiflora was ineffective against the strains tested. After incubation of staphylococci strains in the presence of 1.2 μg.mL(-1) RGE, a significant inhibition of cell growth was observed using both spectrophotometry and ELISA assays. Twelve drugs were evaluated for their antimicrobial effects on culture RGE-treated cells using the disk diffusion method. Penicillin resistant strains became sensitive to the drug after RGE treatment. Furthermore, enterotoxin production by RGE-treated S. aureus was evaluated using a standardized ELISA method. Although staphylococcal LSA 88 bovine strain cells remained viable after exposure to the extract, enterotoxin production was precluded in 20% after RGE treatment. Significant interference in staphylococci cell density, drug sensitivity and enterotoxin secretion was observed after treatment. The study highlights the necessity to find new methods of disease prevention and new antibiotic therapies against staphylococcal infections.
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spelling pubmed-37686402013-09-12 Rauvolfia Grandiflora (Apocynaceae) Extract Interferes With Staphylococcal Density, Enterotoxin Production And Antimicrobial Activity de Almeida Carlos, Lanamar da Silva Amaral, Kenas Aguiar Curcino Vieira, Ivo José Mathias, Leda Braz-Filho, Raimundo Silva Samarão, Solange Vieira-da-Motta, Olney Braz J Microbiol Medical Microbiology Staphylococci bacteria are involved in many human and animal infections and development of alternative antimicrobial drugs against pathogenic bacteria is of great interest to the pharmaceutical industry. This study investigated the in vitro effect of Rauvolfia grandiflora methanol extract (root bark fraction) (RGE) on the density of ATCC strains of Staphylococcus aureus and Staphylococcus epidermidis, and a clinical enterotoxin-producer, S. aureus bovine strain. The alkaloid, isoreserpiline, obtained from dichloromethane extract of R. grandiflora was ineffective against the strains tested. After incubation of staphylococci strains in the presence of 1.2 μg.mL(-1) RGE, a significant inhibition of cell growth was observed using both spectrophotometry and ELISA assays. Twelve drugs were evaluated for their antimicrobial effects on culture RGE-treated cells using the disk diffusion method. Penicillin resistant strains became sensitive to the drug after RGE treatment. Furthermore, enterotoxin production by RGE-treated S. aureus was evaluated using a standardized ELISA method. Although staphylococcal LSA 88 bovine strain cells remained viable after exposure to the extract, enterotoxin production was precluded in 20% after RGE treatment. Significant interference in staphylococci cell density, drug sensitivity and enterotoxin secretion was observed after treatment. The study highlights the necessity to find new methods of disease prevention and new antibiotic therapies against staphylococcal infections. Sociedade Brasileira de Microbiologia 2010 2010-09-01 /pmc/articles/PMC3768640/ /pubmed/24031536 http://dx.doi.org/10.1590/S1517-83822010000300011 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical Microbiology
de Almeida Carlos, Lanamar
da Silva Amaral, Kenas Aguiar
Curcino Vieira, Ivo José
Mathias, Leda
Braz-Filho, Raimundo
Silva Samarão, Solange
Vieira-da-Motta, Olney
Rauvolfia Grandiflora (Apocynaceae) Extract Interferes With Staphylococcal Density, Enterotoxin Production And Antimicrobial Activity
title Rauvolfia Grandiflora (Apocynaceae) Extract Interferes With Staphylococcal Density, Enterotoxin Production And Antimicrobial Activity
title_full Rauvolfia Grandiflora (Apocynaceae) Extract Interferes With Staphylococcal Density, Enterotoxin Production And Antimicrobial Activity
title_fullStr Rauvolfia Grandiflora (Apocynaceae) Extract Interferes With Staphylococcal Density, Enterotoxin Production And Antimicrobial Activity
title_full_unstemmed Rauvolfia Grandiflora (Apocynaceae) Extract Interferes With Staphylococcal Density, Enterotoxin Production And Antimicrobial Activity
title_short Rauvolfia Grandiflora (Apocynaceae) Extract Interferes With Staphylococcal Density, Enterotoxin Production And Antimicrobial Activity
title_sort rauvolfia grandiflora (apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity
topic Medical Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768640/
https://www.ncbi.nlm.nih.gov/pubmed/24031536
http://dx.doi.org/10.1590/S1517-83822010000300011
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