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Differentiation of Bovine Coronavirus (Bcov) Genotypes by a Restriction Enzyme Assay

This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiati...

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Autores principales: Pinheiro de Souza, Sibele, Miyuki Asano, Karen, Lucas Sandri, Thaisa, Nunes de Barros, Iracema, José Richtzenhain, Leonardo, Eduardo Brandão, Paulo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768659/
https://www.ncbi.nlm.nih.gov/pubmed/24031559
http://dx.doi.org/10.1590/S1517-83822010000300034
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author Pinheiro de Souza, Sibele
Miyuki Asano, Karen
Lucas Sandri, Thaisa
Nunes de Barros, Iracema
José Richtzenhain, Leonardo
Eduardo Brandão, Paulo
author_facet Pinheiro de Souza, Sibele
Miyuki Asano, Karen
Lucas Sandri, Thaisa
Nunes de Barros, Iracema
José Richtzenhain, Leonardo
Eduardo Brandão, Paulo
author_sort Pinheiro de Souza, Sibele
collection PubMed
description This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation.
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spelling pubmed-37686592013-09-12 Differentiation of Bovine Coronavirus (Bcov) Genotypes by a Restriction Enzyme Assay Pinheiro de Souza, Sibele Miyuki Asano, Karen Lucas Sandri, Thaisa Nunes de Barros, Iracema José Richtzenhain, Leonardo Eduardo Brandão, Paulo Braz J Microbiol Veterinary Microbiology This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation. Sociedade Brasileira de Microbiologia 2010 2010-09-01 /pmc/articles/PMC3768659/ /pubmed/24031559 http://dx.doi.org/10.1590/S1517-83822010000300034 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Veterinary Microbiology
Pinheiro de Souza, Sibele
Miyuki Asano, Karen
Lucas Sandri, Thaisa
Nunes de Barros, Iracema
José Richtzenhain, Leonardo
Eduardo Brandão, Paulo
Differentiation of Bovine Coronavirus (Bcov) Genotypes by a Restriction Enzyme Assay
title Differentiation of Bovine Coronavirus (Bcov) Genotypes by a Restriction Enzyme Assay
title_full Differentiation of Bovine Coronavirus (Bcov) Genotypes by a Restriction Enzyme Assay
title_fullStr Differentiation of Bovine Coronavirus (Bcov) Genotypes by a Restriction Enzyme Assay
title_full_unstemmed Differentiation of Bovine Coronavirus (Bcov) Genotypes by a Restriction Enzyme Assay
title_short Differentiation of Bovine Coronavirus (Bcov) Genotypes by a Restriction Enzyme Assay
title_sort differentiation of bovine coronavirus (bcov) genotypes by a restriction enzyme assay
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768659/
https://www.ncbi.nlm.nih.gov/pubmed/24031559
http://dx.doi.org/10.1590/S1517-83822010000300034
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