Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies

We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simpli...

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Detalles Bibliográficos
Autores principales: von Groll, Andrea, Martin, Anandi, Portaels, Françoise, da Silva, Pedro Eduardo Almeida, Palomino, Juan Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Medical Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768668/
https://www.ncbi.nlm.nih.gov/pubmed/24031495
http://dx.doi.org/10.1590/S1517-83822010000200006
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author von Groll, Andrea
Martin, Anandi
Portaels, Françoise
da Silva, Pedro Eduardo Almeida
Palomino, Juan Carlos
author_facet von Groll, Andrea
Martin, Anandi
Portaels, Françoise
da Silva, Pedro Eduardo Almeida
Palomino, Juan Carlos
author_sort von Groll, Andrea
collection PubMed
description We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.
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spelling pubmed-37686682013-09-12 Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies von Groll, Andrea Martin, Anandi Portaels, Françoise da Silva, Pedro Eduardo Almeida Palomino, Juan Carlos Braz J Microbiol Medical Microbiology We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate. Sociedade Brasileira de Microbiologia 2010 2010-06-01 /pmc/articles/PMC3768668/ /pubmed/24031495 http://dx.doi.org/10.1590/S1517-83822010000200006 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical Microbiology
von Groll, Andrea
Martin, Anandi
Portaels, Françoise
da Silva, Pedro Eduardo Almeida
Palomino, Juan Carlos
Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies
title Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies
title_full Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies
title_fullStr Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies
title_full_unstemmed Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies
title_short Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies
title_sort growth kinetics of mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies
topic Medical Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768668/
https://www.ncbi.nlm.nih.gov/pubmed/24031495
http://dx.doi.org/10.1590/S1517-83822010000200006
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