A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis

Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study hi...

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Detalles Bibliográficos
Autores principales: Kumar, Gavish, Shankar, Hari, Bisht, Deepa, Sharma, Prashant, Singhal, Neelja, Katoch, V.M., Joshi, Beenu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Medical Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768669/
https://www.ncbi.nlm.nih.gov/pubmed/24031494
http://dx.doi.org/10.1590/S1517-83822010000200005
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author Kumar, Gavish
Shankar, Hari
Bisht, Deepa
Sharma, Prashant
Singhal, Neelja
Katoch, V.M.
Joshi, Beenu
author_facet Kumar, Gavish
Shankar, Hari
Bisht, Deepa
Sharma, Prashant
Singhal, Neelja
Katoch, V.M.
Joshi, Beenu
author_sort Kumar, Gavish
collection PubMed
description Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids.
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spelling pubmed-37686692013-09-12 A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis Kumar, Gavish Shankar, Hari Bisht, Deepa Sharma, Prashant Singhal, Neelja Katoch, V.M. Joshi, Beenu Braz J Microbiol Medical Microbiology Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids. Sociedade Brasileira de Microbiologia 2010 2010-06-01 /pmc/articles/PMC3768669/ /pubmed/24031494 http://dx.doi.org/10.1590/S1517-83822010000200005 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical Microbiology
Kumar, Gavish
Shankar, Hari
Bisht, Deepa
Sharma, Prashant
Singhal, Neelja
Katoch, V.M.
Joshi, Beenu
A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
title A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
title_full A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
title_fullStr A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
title_full_unstemmed A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
title_short A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
title_sort simple and rapid method of sample preparation from culture filtrate of m. tuberculosis for two-dimensional gel electrophoresis
topic Medical Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768669/
https://www.ncbi.nlm.nih.gov/pubmed/24031494
http://dx.doi.org/10.1590/S1517-83822010000200005
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